Evidence map›Paper›PMID 29211103›Full record

ArticleMemorias do Instituto Oswaldo Cruz2018

Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras.

Osbourne Quaye, Sunando Roy, Kunchala Rungsrisuriyachai, Mathew D Esona, Ziqian Xu, Ka Ian Tam, Dina J Castro Banegas, Gloria Rey-Benito, Michael D Bowen

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In one paragraph

Article in Memorias do Instituto Oswaldo Cruz, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Osbourne QuayeCenters for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA.
Sunando RoyCenters for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA.
Kunchala RungsrisuriyachaiCenters for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA.
Mathew D EsonaCenters for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA.
Ziqian XuChina Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, China.
Ka Ian TamCenters for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA.
Dina J Castro BanegasNacional Colonia La Campaña, Tegucigalpa, Honduras.
Gloria Rey-BenitoPan American Health Organization, Washington DC, USA.
Michael D BowenCenters for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAlthough first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains.

objectivesIn this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain.

methodsFor the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0.

findingsThe next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain. MAIN

conclusionsThe genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from multiple reassortment events which were possibly mediated through interspecies transmissions.

Indexed as

Child, PreschoolFecesGenome, ViralGenotypeHondurasHumansMaleReassortant VirusesRNA, ViralRotavirusRotavirus InfectionsRNA, Viral

Identifiers

PMID29211103
PMCPMC5719537

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.