Evidence map›Paper›PMID 29211022›Full record

ArticleCancers2017

Tracking Functional Tumor Cell Subpopulations of Malignant Glioma by Phasor Fluorescence Lifetime Imaging Microscopy of NADH.

Andrew L Trinh, Hongtao Chen, Yumay Chen, Yuanjie Hu, Zhenzhi Li, Eric R Siegel, Mark E Linskey, Ping H Wang, Michelle A Digman, Yi-Hong Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Andrew L TrinhLaboratory for Fluorescence Dynamics and Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA. atrinh2@uci.edu.
Hongtao ChenLaboratory for Fluorescence Dynamics and Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA. hchenuci@gmail.com.
Yumay ChenUC Irvine Diabetes Center and Department of Medicine, University of California, Irvine, CA 92697, USA. yumayc@uci.edu.
Yuanjie HuUC Irvine Brain Tumor Laboratory and Department of Surgery, University of California, Irvine, CA 92697, USA. yuanjieh@uci.edu.
Zhenzhi LiUC Irvine Brain Tumor Laboratory and Department of Surgery, University of California, Irvine, CA 92697, USA. zzrickli@gmail.com.
Eric R SiegelDepartment of Biostatistics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA. SiegelEricR@uams.edu.
Mark E LinskeyUC Irvine Brain Tumor Laboratory and Department of Surgery, University of California, Irvine, CA 92697, USA. mlinskey@uci.edu.
Ping H WangUC Irvine Diabetes Center and Department of Medicine, University of California, Irvine, CA 92697, USA. phwang@uci.edu.
Michelle A DigmanLaboratory for Fluorescence Dynamics and Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA. mdigman@uci.edu.
Yi-Hong ZhouUC Irvine Brain Tumor Laboratory and Department of Surgery, University of California, Irvine, CA 92697, USA. yihongz@uci.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intra-tumoral heterogeneity is associated with therapeutic resistance of cancer and there exists a need to non-invasively identify functional tumor subpopulations responsible for tumor recurrence. Reduced nicotinamide adenine dinucleotide (NADH) is a metabolic coenzyme essential in cellular respiration. Fluorescence lifetime imaging microscopy (FLIM) of NADH has been demonstrated to be a powerful label-free indicator for inferring metabolic states of living cells. Using FLIM, we identified a significant shift towards longer NADH fluorescence lifetimes, suggesting an increase in the fraction of protein-bound NADH, in the invasive stem-like tumor-initiating cell (STIC) subpopulation relative to the tumor mass-forming cell (TMC) subpopulation of malignant gliomas. By applying our previously studied model to transition glioma from a majority of STIC to a majority of TMC in serum-adherent culture conditions following serial passages, we compared changes in NADH states, cellular respirations (oxidative phosphorylation and glycolysis), EGFR expression, and cell-growth speed over passages. We identified a significant positive correlation between free-NADH fraction and cell growth, which was related to an increase of TMC fraction. In comparison, the increase of EGFR and cellular respirations preceded all these changes. In conclusion, FLIM of NADH provides a non-invasive method to monitor the dynamics of tumor heterogeneity before and after treatment.

Indexed as

cellular bioenergeticsfluorescence lifetime imaging microscopy (FLIM)intra-tumoral heterogeneitymalignant gliomaReduced nicotinamide adenine dinucleotide (NADH)stem-like tumor-initiating cellstumor cell subpopulationtumor mass cells

Identifiers

PMID29211022
PMCPMC5742816

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.