Evidence map›Paper›PMID 29209415›Full record

ArticleBiotechnology for biofuels2017

Undefined cellulase formulations hinder scientific reproducibility.

Michael E Himmel, Charles A Abbas, John O Baker, Edward A Bayer, Yannick J Bomble, Roman Brunecky, Xiaowen Chen, Claus Felby, Tina Jeoh, Rajeev Kumar and 5 more

Abstract readEditorial
In one paragraph

Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Effects ofBiotechnology for biofuels · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Michael E HimmelBiosciences Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
Charles A AbbasDepartment of Food Science and Human Nutrition, University of Illinois, 905 S. Goodwin Ave., Urbana, IL 61801 USA.
John O BakerBiosciences Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
Edward A BayerDepartment of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
Yannick J BombleBiosciences Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
Roman BruneckyBiosciences Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
Xiaowen ChenNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
Claus FelbyDepartment of Geosciences and Natural Resource Management, University of Copenhagen, Rolighedsvej 23, 1958 Frederiksberg C, Denmark.
Tina JeohDepartment of Biological and Agricultural Engineering, University of California Davis, Davis, CA 95616 USA.
Rajeev KumarBourns College of Engineering Center for Environmental Research and Technology, University of California, Riverside, 900 University Ave., Riverside, CA 92521 USA.
Barry V McClearyMegazyme, Irishtown, Bray, Co. Wicklow, A98 YV29 Ireland.
Brett I PletschkeDepartment of Biochemistry and Microbiology, Rhodes University, PO Box 94, Grahamstown, 6140 South Africa.
Melvin P TuckerDepartment of Food Science and Human Nutrition, University of Illinois, 905 S. Goodwin Ave., Urbana, IL 61801 USA.
Charles E WymanBourns College of Engineering Center for Environmental Research and Technology, University of California, Riverside, 900 University Ave., Riverside, CA 92521 USA.
Stephen R DeckerBiosciences Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations.

Indexed as

AssaysBiofuelsCellulaseCelluloseCommercial cellulase formulations

Identifiers

PMID29209415
PMCPMC5704559

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.