ArticleBiotechnology for biofuels2017
Quantitative in vivo phosphoproteomics reveals reversible signaling processes during nitrogen starvation and recovery in the biofuel model organism
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Increasing lipid accumulation in Chlamydomonas by serial knocking out of DYRKP1 kinase and ADP-glucose pyrophosphorylase.Microbial cell factories · 2025Article
- Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth.Plant biotechnology journal · 2025Article
- The microbial phosphorus cycle in aquatic ecosystems.Nature reviews. Microbiology · 2025Review
- Target of Rapamycin is a crucial regulator of photosynthesis and nutrient metabolism partitioning in Nannochloropsis gaditana.Biotechnology for biofuels and bioproducts · 2025Article
- Evolutionary conservation and metabolic significance of autophagy in algae.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algae Chlorella sorokiniana and Chlamydomonas reinhardtii.BMC plant biology · 2024Article
- Opportunity for genome engineering to enhance phosphate homeostasis in crops.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- From Feasting to Fasting: The Arginine Pathway as a Metabolic Switch in Nitrogen-DeprivedCells · 2023Review
- Article
- Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM).Methods in molecular biology (Clifton, N.J.) · 2022Article
- The TOR-Auxin Connection Upstream of Root Hair Growth.Plants (Basel, Switzerland) · 2021Review
- Development of New Antiproliferative Compound against Human Tumor Cells from the Marine MicroalgaeInternational journal of molecular sciences · 2020Article
- Target of Rapamycin in Control of Autophagy: Puppet Master and Signal Integrator.International journal of molecular sciences · 2020Review
- The Plant Target of Rapamycin: A Conduc TOR of Nutrition and Metabolism in Photosynthetic Organisms.Genes · 2020Review
- Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green AlgaInternational journal of molecular sciences · 2020Article
- Fatty acid synthesis by Chlamydomonas reinhardtii in phosphorus limitation.Journal of bioenergetics and biomembranes · 2020Article
- Phosphorus Availability Regulates TORC1 Signaling via LST8 in Chlamydomonas.The Plant cell · 2020Article
- Evaluation of linear models and missing value imputation for the analysis of peptide-centric proteomics.BMC bioinformatics · 2019Article
- Genome-wide identification and characterization of CKIN/SnRK gene family in Chlamydomonas reinhardtii.Scientific reports · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNitrogen deprivation and replenishment induces massive changes at the physiological and molecular level in the green alga
resultsTo decipher the signaling pathways involved in this dynamic process, we applied a label-free in vivo shotgun phosphoproteomics analysis on nitrogen-depleted and recovered samples. 1227 phosphopeptides belonging to 732 phosphoproteins were identified and quantified. 470 phosphopeptides showed a significant change across the experimental set-up. Multivariate statistics revealed the reversible phosphorylation process and the time/condition-dependent dynamic rearrangement of the phosphoproteome. Protein-protein interaction analysis of differentially regulated phosphoproteins identified protein kinases and phosphatases, such as DYRKP and an AtGRIK1 orthologue, called CDPKK2, as central players in the coordination of translational, photosynthetic, proteomic and metabolomic activity. Phosphorylation of RPS6, ATG13, and NNK1 proteins points toward a specific regulation of the TOR pathway under nitrogen deprivation. Differential phosphorylation pattern of several eukaryotic initiation factor proteins (EIF) suggests a major control on protein translation and turnover.
conclusionThis work provides the first phosphoproteomics dataset obtained for
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