ArticleScientific reports2017
Multiplex three-dimensional optical mapping of tumor immune microenvironment.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
- NicheProt: Cell-type-resolved proteomics of tissue compartments.Science advances · 2026Article
- Integrative single-cell, spatial, and bulk transcriptomics reveal an FMR1-FTO axis linked to the immune-excluded phenotype in gastric cancer.Frontiers in immunology · 2026Article
- Article
- Immune and non-immune cell fencing of tumor cells is a widespread and functionally relevant spatial pattern in solid cancers.Computational and structural biotechnology journal · 2025Article
- Context-dependent roles for autophagy in myeloid cells in tumor progression.bioRxiv : the preprint server for biology · 2024Article
- Transmural Flow Upregulates PD-L1 Expression in Microvascular Networks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Increased Multiplexity in Optical Tissue Clearing-Based Three-Dimensional Immunofluorescence Microscopy of the Tumor Microenvironment by Light-Emitting Diode Photobleaching.Laboratory investigation; a journal of technical methods and pathology · 2024Article
- Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- Three-dimensional spatial quantitative analysis of cardiac lymphatics in the mouse heart.Microcirculation (New York, N.Y. : 1994) · 2023Article
- Locally sourced: site-specific immune barriers to metastasis.Nature reviews. Immunology · 2023Review
- Multiresolution 3D Optical Mapping of Immune Cell Infiltrates in Mouse Asthmatic Lung.American journal of respiratory cell and molecular biology · 2023Article
- Nontoxic Fluorescent Nanoprobes for Multiplexed Detection and 3D Imaging of Tumor Markers in Breast Cancer.Pharmaceutics · 2023Review
- Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time.Frontiers in immunology · 2023Review
- Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D.Nature protocols · 2022Review
- FN3 linked nanobubbles as a targeted contrast agent for US imaging of cancer-associated human PD-L1.Journal of controlled release : official journal of the Controlled Release Society · 2022Article
- LIGHT enhanced bispecific antibody armed T-cells to treat immunotherapy resistant colon cancer.Oncogene · 2022Article
- Computer-assisted three-dimensional quantitation of programmed death-ligand 1 in non-small cell lung cancer using tissue clearing technology.Journal of translational medicine · 2022Article
- A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases.Cellular and molecular gastroenterology and hepatology · 2022Article
- Tissue clearing to examine tumour complexity in three dimensions.Nature reviews. Cancer · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Recent developments in optical tissue clearing and microscopic imaging have advanced three-dimensional (3D) visualization of intact tissues and organs at high resolution. However, to expand applications to oncology, critical limitations of current methods must be addressed. Here we describe transparent tissue tomography (T3) as a tool for rapid, three-dimensional, multiplexed immunofluorescent tumor imaging. Cutting tumors into sub-millimeter macrosections enables simple and rapid immunofluorescence staining, optical clearing, and confocal microscope imaging. Registering and fusing macrosection images yields high resolution 3D maps of multiple tumor microenvironment components and biomarkers throughout a tumor. The 3D maps can be quantitatively evaluated by automated image analysis. As an application of T3, 3D mapping and analysis revealed a heterogeneous distribution of programmed death-ligand 1 (PD-L1) in Her2 transgenic mouse mammary tumors, with high expression limited to tumor cells at the periphery and to CD31
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.