ArticleMicrobial genomics2017
Suspected cases of intracontinental Burkholderia pseudomallei sequence type homoplasy resolved using whole-genome sequencing.
Article in Microbial genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Comparative genomic analysis of paired clinical isolates from a patient with recurrent melioidosis reveals a low within-host mutation rate.Journal of medical microbiology · 2025Article
- Analysis of fine-scale phylogeny of Burkholderia pseudomallei in relation to regional geography and drug susceptibility in Thailand.Scientific reports · 2024Article
- Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a.PLoS neglected tropical diseases · 2022Article
- Development and Validation of a Burkholderia pseudomallei Core Genome Multilocus Sequence Typing Scheme To Facilitate Molecular Surveillance.Journal of clinical microbiology · 2021Article
- Genomics of an endemic cystic fibrosis Burkholderia multivorans strain reveals low within-patient evolution but high between-patient diversity.PLoS pathogens · 2021Article
- Using Land Runoff to Survey the Distribution and Genetic Diversity ofApplied and environmental microbiology · 2021Article
- Article
- Emergence of Burkholderia pseudomallei Sequence Type 562, Northern Australia.Emerging infectious diseases · 2021Article
- Article
- Myanmar Burkholderia pseudomallei strains are genetically diverse and originate from Asia with phylogenetic evidence of reintroductions from neighbouring countries.Scientific reports · 2020Article
- Comparative genomics confirms a rare melioidosis human-to-human transmission event and reveals incorrect phylogenomic reconstruction due to polyclonality.Microbial genomics · 2020Article
- An Evolutionary Arms Race BetweenFrontiers in microbiology · 2020Review
- Melioidosis fatalities in captive slender-tailed meerkats (Suricata suricatta): combining epidemiology, pathology and whole-genome sequencing supports variable mechanisms of transmission with one health implications.BMC veterinary research · 2019Article
- Whole-Genome Sequence of Burkholderia pseudomallei Strain HNBP001, Isolated from a Melioidosis Patient in Hainan, China.Microbiology resource announcements · 2019Article
- A cluster of melioidosis infections in hatchling saltwater crocodiles (Microbial genomics · 2019Article
- Tracing the environmental footprint of the Burkholderia pseudomallei lipopolysaccharide genotypes in the tropical "Top End" of the Northern Territory, Australia.PLoS neglected tropical diseases · 2019Article
- Molecular Signatures of Non-typeableFrontiers in microbiology · 2019Article
- Molecular analysis of clinical Burkholderia pseudomallei isolates from southwestern coastal region of India, using multi-locus sequence typing.PLoS neglected tropical diseases · 2018Article
- Melioidosis in Africa: Time to Uncover the True Disease Load.Tropical medicine and infectious disease · 2018Review
- Melioidosis: An Australian Perspective.Tropical medicine and infectious disease · 2018Review
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8 authors.
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Abstract
Burkholderia pseudomallei is a Gram-negative environmental bacterium that causes melioidosis, a disease of high mortality in humans and animals. Multilocus sequence typing (MLST) is a popular and portable genotyping method that has been used extensively to characterise the genetic diversity of B. pseudomallei populations. MLST has been central to our understanding of the underlying phylogeographical signal present in the B. pseudomallei genome, revealing distinct populations on both the intra- and the inter-continental level. However, due to its high recombination rate, it is possible for B. pseudomallei isolates to share the same multilocus sequence type (ST) despite being genetically and geographically distinct, with two cases of 'ST homoplasy' recently reported between Cambodian and Australian B. pseudomallei isolates. This phenomenon can dramatically confound conclusions about melioidosis transmission patterns and source attribution, a critical issue for bacteria such as B. pseudomallei that are of concern due to their potential for use as bioweapons. In this study, we used whole-genome sequencing to identify the first reported instances of intracontinental ST homoplasy, which involved ST-722 and ST-804 B. pseudomallei isolates separated by large geographical distances. In contrast, a third suspected homoplasy case was shown to be a true long-range (460 km) dispersal event between a remote Australian island and the Australian mainland. Our results show that, whilst a highly useful and portable method, MLST can occasionally lead to erroneous conclusions about isolate origin and disease attribution. In cases where a shared ST is identified between geographically distant locales, whole-genome sequencing should be used to resolve strain origin.
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