ReviewMicrobial biotechnology2018
Maintenance and assessment of cell viability in formulation of non-sporulating bacterial inoculants.
Review in Microbial biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of sublethal stress application on the survival of bacterial inoculants: a systematic review.Archives of microbiology · 2023Pooled it
- Microbial Biostimulants for Drought Resilience in Plants: Mechanisms, Plant-Microbiome Interactions, and Sustainable Agricultural Applications.Plants (Basel, Switzerland) · 2026Review
- Controlled-release gel encapsulation: an emerging technology for delivering beneficial microorganisms in sustainable plant disease management.Crop health · 2026Review
- Towards an Omics-Guided Framework for Microbial Biological Control of Fungal and Oomycete Diseases inInternational journal of molecular sciences · 2026Review
- Use of beneficial microorganisms in bean cultivation: strategies employed to increase productivity.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Review
- Valorization ofBiotech (Basel (Switzerland)) · 2026Article
- Review
- Bio-inspired core-shell microcapsules enhance plant salinity tolerance by improving stability and survival of non-spore bacteria.Nature communications · 2026Article
- Post-Inoculation Drying and Storage Effects on HAB Viability and Nutrient Retention in Biochar.Biotech (Basel (Switzerland)) · 2026Article
- Biocontrol ofFrontiers in plant science · 2026Article
- Zeolite-amended peat carriers improve long-term survival ofAIMS microbiology · 2026Article
- Silk-trehalose seed coating technology preservesFrontiers in plant science · 2026Article
- Fit for purpose: evaluation and evolution of agricultural bioinoculants across production landscapes.ISME communications · 2026Review
- A novel solid-state, multi-layered biodegradable microbial inoculant system for rice straw composting: biocapsule design, characterization, and performance evaluation.Frontiers in microbiology · 2026Article
- Biocontrol of Bacterial Seedling Rot in Rice Plants Using Bacillus velezensis JBCS608 and Its Formulated Products.The plant pathology journal · 2025Article
- Improving olive farming with microbial biostimulants: benefits, challenges and opportunities.World journal of microbiology & biotechnology · 2025Review
- Recent Advances in Bioinoculant Formulations and Their Shelf-Life: A Review.Current microbiology · 2025Review
- Evolving a plant-beneficial bacterium in soil vs. nutrient-rich liquid culture has contrasting effects on in-soil fitness.Applied and environmental microbiology · 2025Article
- Seed banking impacts native Acacia ulicifolia seed microbiome composition and function.Environmental microbiome · 2025Article
- Root colonization dynamics of alginate encapsulated rhizobacteria: implications forAIMS microbiology · 2025Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of beneficial, plant-associated microorganisms is a sustainable approach to improving crop performance in agriculture. However, microbial inoculants are often susceptible to prolonged periods of storage and deleterious environmental factors, which negatively impact their viability and ultimately limit efficacy in the field. This particularly concerns non-sporulating bacteria. To overcome this challenge, the availability of protective formulations is crucial. Numerous parameters influence the viability of microbial cells, with drying procedures generally being among the most critical ones. Thus, technological advances to attenuate the desiccation stress imposed on living cells are key to successful formulation development. In this review, we discuss the core aspects important to consider when aiming at high cell viability of non-sporulating bacteria to be applied as microbial inoculants in agriculture. We elaborate the suitability of commonly applied drying methods (freeze-drying, vacuum-drying, spray-drying, fluidized bed-drying, air-drying) and potential measures to prevent cell damage from desiccation (externally applied protectants, stress pre-conditioning, triggering of exopolysaccharide secretion, 'helper' strains). Furthermore, we point out methods for assessing bacterial viability, such as colony counting, spectrophotometry, microcalorimetry, flow cytometry and viability qPCR. Choosing appropriate technologies for maintenance of cell viability and evaluation thereof will render formulation development more efficient. This in turn will aid in utilizing the vast potential of promising, plant beneficial bacteria as sustainable alternatives to standard agrochemicals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.