Evidence map›Paper›PMID 29205767›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2017

Atomic mass tag of bismuth-209 for increasing the immunoassay multiplexing capacity of mass cytometry.

Guojun Han, Shih-Yu Chen, Veronica D Gonzalez, Eli R Zunder, Wendy J Fantl, Garry P Nolan

Open access · bronzeAbstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 51 citations in OpenAlex.

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  13. CyTOFFrontiers in immunology · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Guojun HanBaxter Laboratory for Stem Cell Biology Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford California.
Shih-Yu ChenBaxter Laboratory for Stem Cell Biology Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford California.
Veronica D GonzalezBaxter Laboratory for Stem Cell Biology Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford California.
Eli R ZunderDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia.
Wendy J FantlStanford Comprehensive Cancer Institute and Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford California.
Garry P NolanBaxter Laboratory for Stem Cell Biology Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford California.
Stanford University · USBaxter (United States) · USUniversity of Virginia · US

Funding

Using a tonsil organoid system to probe conditions for the induction of protective antibody and T cell responses to influenza.U19AI057229 · NIAID · STANFORD UNIVERSITY · PI Mark Morris Davis · 2003 to 2026
$88.5M
Systems Biology CoreU19AI100627 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI ULEVITCH, RICHARD J · 2012 to 2021
$49.1M
(PQD2)New Biomarkers and Pathways to Enhance Cure in Ovarian CancersR01CA184968 · NCI · STANFORD UNIVERSITY · PI FANTL, WENDY JANE, SIKIC, BRANIMIR I · 2014 to 2017
$2.0M
Highly multiplexed ion-beam tissue molecular imaging with sub-micron resolutionR33CA183654 · NCI · STANFORD UNIVERSITY · PI NOLAN, GARRY P · 2014 to 2016
$1.2M
Integrating mass cytometric and transcriptomic profiles of solid tumorsR33CA183692 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WEISS, WILLIAM A · 2014 to 2016
$1.2M
NCI NIH HHS R01 CA184968NCI NIH HHS R33 CA183654NCI NIH HHS R33 CA183692NIAID NIH HHS U19 AI057229NIAID NIH HHS U19 AI100627
6 · The paper itself

Abstract

Mass cytometry (or CyTOF) is an atomic mass spectrometry-based single-cell immunoassay technology, which has provided an increasingly systematic and sophisticated view in basic biological and clinical studies. Using elemental reporters composed of stable heavy metal isotopes, more than 50 cellular parameters are measured simultaneously. However, this current multiplexing does not meet the theoretical capability of CyTOF instrumentation with 135 detectable channels, primarily due to the limitation of available chemistries for conjugating elemental mass tags to affinity reagents. To address this issue, we develop herein additional metallic mass tag based on bismuth-209 (

Indexed as

Killer Cells, NaturalAntibodies, MonoclonalBismuthFlow CytometryHumansImmunoassayImmunoconjugatesMass SpectrometrySingle-Cell AnalysisAntibodies, MonoclonalBismuthBismuth-209Immunoconjugatesantibody conjugationbismuthcell cycle measurementelemental mass tagslabeling efficiencymass cytometryNK cell phenotyping

Identifiers

PMID29205767
PMCPMC5802970
OpenAlexW2771254572

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.