Evidence map›Paper›PMID 29204685›Full record

ArticleLung2018

Methylene Blue Protects the Isolated Rat Lungs from Ischemia-Reperfusion Injury by Attenuating Mitochondrial Oxidative Damage.

Wen-Fang Tian, Si Zeng, Qiong Sheng, Jun-Liang Chen, Ping Weng, Xiao-Tong Zhang, Jia-Jia Yuan, Qing-Feng Pang, Zhi-Qiang Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Lung, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Trial
  2. Methylene blue in the critical care setting.World journal of critical care medicine · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Wen-Fang TianWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China.
Si ZengDepartment of Anesthesiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.
Qiong ShengWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China.
Jun-Liang ChenWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China.
Ping WengWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China.
Xiao-Tong ZhangWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China.
Jia-Jia YuanWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China.
Qing-Feng PangWuxi School of Medicine, Jiangnan University, No.1800 Lihu Road, 214122, Wuxi, China. qfpang@jiangnan.edu.cn.ORCID 0000-0003-0679-4780
Zhi-Qiang WangDepartment of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Jiangnan University, No. 200, Huihe Road, 214062, Wuxi, China. wxsywzq@163.com.
Jiangnan University · CNSichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital · CN

Funding

Fundamental Research Funds for the Central Universities JUSRP51412BJiangsu Province Graduate Student Research Innovation Project KYLX15_1196Jiangsu Province Graduate Student Research Innovation Project KYZZ16_0312Jiangsu Province Natural Science Foundation BK20161141National Natural Science Foundation of China 81270126National University Students Innovation Training 201610295074The Science Funds for Health and Family Planning Commission of Sichuan Province 150234
6 · The paper itself

Abstract

introductionImpaired mitochondrial function is a key factor attributing to the lung ischemia reperfusion injury (LIRI). Methylene blue (MB) has been reported to attenuate brain and renal ischemia-reperfusion injury. We hypothesized that MB also could have a protective effect against LIRI by preventing mitochondrial oxidative damage.

methodsIsolated rat lungs were assigned to the following four groups (n = 6): a sham group: perfusion for 105 min without ischemia; I/R group: shutoff of perfusion and ventilation for 45 min followed by reperfusion for 60 min; and I/R + MB group and I/R + glutathione (GSH) group: 2 mg/kg MB or 4 μM glutathione were intraperitoneally administered for 2 h, and followed by 45 min of ischemia and 60 min of reperfusion.

resultsMB lessened pulmonary dysfunction and severe histological injury induced by ischemia-reperfusion injury. MB reduced the production of reactive oxygen species and malondialdehyde and enhanced the activity of superoxide dismutase. MB also suppressed the opening of the mitochondrial permeability transition pore and partly preserved mitochondrial membrane potential. Moreover, MB inhibited the release of cytochrome c from the mitochondria into the cytosol and decreased apoptosis. Additionally, MB downregulated the mRNA expression levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-18).

conclusionMB protects the isolated rat lungs against ischemia-reperfusion injury by attenuating mitochondrial damage.

Indexed as

AnimalsApoptosisBiological TransportCytochromes cCytokinesEnzyme InhibitorsLungMaleMalondialdehydeMembrane Potential, MitochondrialMethylene BlueMitochondriaMitochondrial Membrane Transport ProteinsMitochondrial Permeability Transition PoreOxidative StressRatsCytochromes cCytokinesEnzyme InhibitorsMalondialdehydeMethylene BlueMitochondrial Membrane Transport ProteinsMitochondrial Permeability Transition PoreReactive Oxygen SpeciesSuperoxide DismutaseIsolated lung reperfusionLung ischemia–reperfusion injuryMethylene blueMitochondrial damage

Identifiers

PMID29204685
OpenAlexW2771214544

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.