ArticleCellular and molecular gastroenterology and hepatology2017
Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.
Article in Cellular and molecular gastroenterology and hepatology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
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The trial behind it
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Who cites it
147 citing papers in PubMed, 229 citations in OpenAlex.
- A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels.Nature biotechnology · 2026Article
- Equine mesenchymal stromal cells dual-primed with TGF-β1 and IL-1β or TNF-α improve epithelial healing.Research square · 2026Article
- In vitro modeling of inflammatory bowel diseases using a newly developed immunocompetent colon epithelial monolayer co-culture model.npj biomedical innovations · 2026Article
- Development and characterization of immortalized mouse intestinal epithelial cell lines.Scientific reports · 2026Article
- A multidimensional platform to support intestinal epithelial and immune cell co-culture.Frontiers in nutrition · 2026Article
- A Shared Framework for Gastrointestinal Organoid Research: It's Time.Cellular and molecular gastroenterology and hepatology · 2026Article
- AnBiofabrication · 2025Article
- Forward genetic screening in engineered colorectal cancer organoids identifies regulators of metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Coupling mathematical modeling with a novel human intestinal stem cell system to understand feedback regulation during planar cell polarity.bioRxiv : the preprint server for biology · 2025Article
- A scalable long-termbioRxiv : the preprint server for biology · 2025Article
- Article
- Organoids in parasitology: a game-changer for studying host-nematode interactions.Parasitology · 2025Review
- Comprehensive gene expression analysis of organoid-derived healthy human colonic epithelium and cancer cell line stimulated with live probiotic bacteria.Scientific reports · 2025Article
- Human mesofluidic intestinal model for studying transport of drug carriers and bacteria through a live mucosal barrier.Lab on a chip · 2025Article
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- High-throughput assay for predicting diarrhea risk using a 2D human intestinal stem cell-derived model.Toxicology in vitro : an international journal published in association with BIBRA · 2025Article
- Porcine intestinal organoids cultured in an organ-on-a-chip microphysiological system.Biochemistry and biophysics reports · 2025Article
- Leveraging Organ-on-Chip Models to Investigate Host-Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease.Advanced healthcare materials · 2025Review
- Single-cell transcriptomics predict novel potential regulators of acute epithelial restitution in the ischemia-injured intestine.American journal of physiology. Gastrointestinal and liver physiology · 2025Article
- Morphogenesis of bacterial cables in polymeric environments.Science advances · 2025Article
87 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
BACKGROUND &
aimsThree-dimensional organoid culture has fundamentally changed the in vitro study of intestinal biology enabling novel assays; however, its use is limited because of an inaccessible luminal compartment and challenges to data gathering in a three-dimensional hydrogel matrix. Long-lived, self-renewing 2-dimensional (2-D) tissue cultured from primary colon cells has not been accomplished.
methodsThe surface matrix and chemical factors that sustain 2-D mouse colonic and human rectal epithelial cell monolayers with cell repertoires comparable to that in vivo were identified.
resultsThe monolayers formed organoids or colonoids when placed in standard Matrigel culture. As with the colonoids, the monolayers exhibited compartmentalization of proliferative and differentiated cells, with proliferative cells located near the peripheral edges of growing monolayers and differentiated cells predominated in the central regions. Screening of 77 dietary compounds and metabolites revealed altered proliferation or differentiation of the murine colonic epithelium. When exposed to a subset of the compound library, murine organoids exhibited similar responses to that of the monolayer but with differences that were likely attributable to the inaccessible organoid lumen. The response of the human primary epithelium to a compound subset was distinct from that of both the murine primary epithelium and human tumor cells.
conclusionsThis study demonstrates that a self-renewing 2-D murine and human monolayer derived from primary cells can serve as a physiologically relevant assay system for study of stem cell renewal and differentiation and for compound screening. The platform holds transformative potential for personalized and precision medicine and can be applied to emerging areas of disease modeling and microbiome studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.