ArticleScientific reports2017
Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
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- Patient-Derived Organoids as Therapy Screening Platforms in Cancer Patients.Advanced healthcare materials · 2024Review
- The importance of 3D fibre architecture in cancer and implications for biomaterial model design.Nature reviews. Cancer · 2024Review
- Therapeutic blockade of ER stress and inflammation prevents NASH and progression to HCC.Science advances · 2023Article
- A first-in-class pan-lysyl oxidase inhibitor impairs stromal remodeling and enhances gemcitabine response and survival in pancreatic cancer.Nature cancer · 2023Article
- IGFBP2 secretion by mammary adipocytes limits breast cancer invasion.Science advances · 2023Article
- Temporal profiling of the breast tumour microenvironment reveals collagen XII as a driver of metastasis.Nature communications · 2022Article
- Xenoimplant of Collagen Matrix Scaffold in Liver Tissue as a Niche for Liver Cells.Frontiers in medicine · 2022Article
- A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept.Scientific reports · 2021Article
- Stromal cell diversity associated with immune evasion in human triple-negative breast cancer.The EMBO journal · 2020Article
- Sea Cucumber Derived Type I Collagen: A Comprehensive Review.Marine drugs · 2020Review
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- The Mini-Organo: A rapid high-throughput 3D coculture organotypic assay for oncology screening and drug development.Cancer reports (Hoboken, N.J.) · 2020Article
- New Frontiers for Biofabrication and Bioreactor Design in Microphysiological System Development.Trends in biotechnology · 2019Review
- CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan.Nature communications · 2019Article
- Review: Synthetic scaffolds to control the biochemical, mechanical, and geometrical environment of stem cell-derived brain organoids.APL bioengineering · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organotypic co-cultures bridge the gap between standard two-dimensional culture and mouse models. Such assays increase the fidelity of pre-clinical studies, to better inform lead compound development and address the increasing attrition rates of lead compounds within the pharmaceutical industry, which are often a result of screening in less faithful two-dimensional models. Using large-scale acid-extraction techniques, we demonstrate a step-by-step process to isolate collagen I from commercially available animal byproducts. Using the well-established rat tail tendon collagen as a benchmark, we apply our novel kangaroo tail tendon collagen as an alternative collagen source for our screening-ready three-dimensional organotypic co-culture platform. Both collagen sources showed equal applicability for invasive, proliferative or survival assessment of well-established cancer models and clinically relevant patient-derived cancer cell lines. Additional readouts were also demonstrated when comparing these alternative collagen sources for stromal contributions to stiffness, organization and ultrastructure via atomic force microscopy, second harmonic generation imaging and scanning electron microscopy, among other vital biological readouts, where only minor differences were found between the preparations. Organotypic co-cultures represent an easy, affordable and scalable model to investigate drug responses within a physiologically relevant 3D platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.