ArticleProceedings of the National Academy of Sciences of the United States of America2017
Electrostatic confinement and manipulation of DNA molecules for genome analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- DOM: Dual Optical Mapping Integrating Sequence-Specific Barcodes and A/T Density Profiles.Small methods · 2026Article
- Developing an insert to protect large DNA molecules during cell lysis.BioTechniques · 2025Article
- Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal-Free Electro-Stain Composed of DNA-Binding Proteins and Synthetic Polymers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Concentration of lambda concatemers using a 3D printed device.Electrophoresis · 2023Article
- DNA Visualization Using Fluorescent Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- 21 Fluorescent Protein-Based DNA Staining Dyes.Molecules (Basel, Switzerland) · 2022Article
- Multidimensional insights into the repeated electromagnetic field stimulation and biosystems interaction in aging and age-related diseases.Journal of biomedical science · 2022Review
- Hydrogen Bonding in Natural and Unnatural Base Pairs-A Local Vibrational Mode Study.Molecules (Basel, Switzerland) · 2021Article
- Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer.Scientific reports · 2019Article
- Development of 3D printed mesofluidic devices to elute and concentrate DNA.Electrophoresis · 2019Article
- A simple dialysis device for large DNA molecules.BioTechniques · 2019Article
- Microscale Objects via Restructuring of Large, Double-Stranded DNA Molecules.ACS applied materials & interfaces · 2018Article
- TAMRA-polypyrrole for A/T sequence visualization on DNA molecules.Nucleic acids research · 2018Article
- Measuring the wall depletion length of nanoconfined DNA.The Journal of chemical physics · 2018Article
- Distribution of label spacings for genome mapping in nanochannels.Biomicrofluidics · 2018Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Very large DNA molecules enable comprehensive analysis of complex genomes, such as human, cancer, and plants because they span across sequence repeats and complex somatic events. When physically manipulated, or analyzed as single molecules, long polyelectrolytes are problematic because of mechanical considerations that include shear-mediated breakage, dealing with the massive size of these coils, or the length of stretched DNAs using common experimental techniques and fluidic devices. Accordingly, we harness analyte "issues" as exploitable advantages by our invention and characterization of the "molecular gate," which controls and synchronizes formation of stretched DNA molecules as DNA dumbbells within nanoslit geometries. Molecular gate geometries comprise micro- and nanoscale features designed to synergize very low ionic strength conditions in ways we show effectively create an "electrostatic bottle." This effect greatly enhances molecular confinement within large slit geometries and supports facile, synchronized electrokinetic loading of nanoslits, even without dumbbell formation. Device geometries were considered at the molecular and continuum scales through computer simulations, which also guided our efforts to optimize design and functionalities. In addition, we show that the molecular gate may govern DNA separations because DNA molecules can be electrokinetically triggered, by varying applied voltage, to enter slits in a size-dependent manner. Lastly, mapping the
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