Evidence map›Paper›PMID 29203477›Full record

ArticleThe Journal of general physiology2018

PnuT uses a facilitated diffusion mechanism for thiamine uptake.

Michael Jaehme, Rajkumar Singh, Alisa A Garaeva, Ria H Duurkens, Dirk-Jan Slotboom

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Structural and Functional Characterization of NadR fromMolecules (Basel, Switzerland) · 2020
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Michael JaehmeGroningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, Netherlands.
Rajkumar SinghGroningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, Netherlands.ORCID 0000-0001-8101-2131
Alisa A GaraevaGroningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, Netherlands.ORCID 0000-0002-7394-8981
Ria H DuurkensGroningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, Netherlands.
Dirk-Jan SlotboomGroningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, Netherlands d.j.slotboom@rug.nl.ORCID 0000-0002-5804-9689
University of Groningen · NL

Funding

European Research Council 282083
6 · The paper itself

Abstract

Membrane transporters of the bacterial pyridine nucleotide uptake (Pnu) family mediate the uptake of various B-type vitamins. For example, the PnuT transporters have specificity for vitamin B1 (thiamine). It has been hypothesized that Pnu transporters are facilitators that allow passive transport of the vitamin substrate across the membrane. Metabolic trapping by phosphorylation would then lead to accumulation of the transported substrates in the cytoplasm. However, experimental evidence for such a transport mechanism is lacking. Here, to determine the mechanism of thiamine transport, we purify PnuT

Indexed as

Bacterial ProteinsBinding SitesDiffusionMembrane Transport ProteinsProtein BindingShewanellaThiamineBacterial ProteinsMembrane Transport ProteinsThiamine

Identifiers

PMID29203477
PMCPMC5749112
OpenAlexW2773529376

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.