ReviewBiomolecular detection and quantification2017
qPCR primer design revisited.
Review in Biomolecular detection and quantification, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
121 citing papers in PubMed.
- The effect of combined oral contraceptive pills on angiogenesis in endometriotic lesions.Hormones (Athens, Greece) · 2025Trial
- Validation of mitochondrialFood chemistry. Molecular sciences · 2026Article
- Silver nanoparticles enhance organogenesis, total flavonoid and anthocyanin contents inBiotechnology reports (Amsterdam, Netherlands) · 2026Article
- GC-rich regions affect amplification efficiency of neighboring amplicons in qPCR and digital PCR.Scientific reports · 2026Article
- Development and Validation of a Viability RT-qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus (SVCV).Journal of fish diseases · 2026Article
- Analytical Validation and Preliminary Diagnostic Performance Evaluation of GenoPATHX™ Multiplex qPCR for Quantitative Detection of KeyPathogens (Basel, Switzerland) · 2026Article
- GEAR Genomics: a user-friendly, open-source web platform enabling interactive genomic analysis for molecular biologists.Nucleic acids research · 2026Article
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Comprehensive characterization of the human neural stem cell line HNSC.100 as a versatile model for neurobiological research.Biochemistry and biophysics reports · 2026Article
- Reporting quality of quantitative polymerase chain reaction (qPCR) methods in scientific publications.Research integrity and peer review · 2026Article
- A Sensitive Real-Time PCR-Based Assay for the Identification ofJournal of fungi (Basel, Switzerland) · 2026Article
- Rumen microbiota inoculation indicates collaborative mechanisms enhancing propionate supply to alleviate weaning stress in lambs.Microbiome · 2026Article
- Mycobiont-specific primers for lichenized fungal genusMycoKeys · 2026Article
- Insights ofInternational journal of microbiology · 2026Article
- Molecular monitoring and multiplex qPCR-based detection ofFrontiers in insect science · 2026Article
- Optimization and validation of the real-time polymerase chain reaction protocol for porcine gelatin detection in processed food and cosmetic products.Open veterinary journal · 2026Article
- The flavonoid isoliquiritigenin promotes lifespan extension of Caenorhabditis elegans through the transcription factor DAF-16 and aquaporin-2.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas · 2026Article
- Trait Mapping Utilizing a Newly Constructed Genome for Allohexaploid Invasive Eurasian Watermilfoil (Evolutionary applications · 2026Article
- Saffron as a natural modulator of reverse cholesterol transport genes in atherosclerotic rabbits, with molecular docking insights.Scientific reports · 2025Article
- Lactulose selectively stimulates members of the gut microbiota, as determined by multi-modal activity-based sorting.Gut microbes · 2025Article
61 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative detection of amplification targets. Despite the framework provided by the MIQE guidelines and the accessibility of wide-ranging support from peer-reviewed publications, books and online sources as well as commercial companies, the design of many published assays continues to be less than optimal: primers often lack intended specificity, can form dimers, compete with template secondary structures at the primer binding sites or hybridise only within a narrow temperature range. We present an overview of the main steps in the primer design workflow, with data that illustrate some of the unexpected variability that often occurs when theory is translated into practice. We also strongly urge researchers to report as much information about their assays as possible in their publications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.