ArticlePeerJ2017
Evaluation of potential reference genes for real-time qPCR analysis in a biparental beetle,
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Article
- Assessment of housekeeping genes stability for gene transcription regulation analysis of Spodoptera littoralis (Lepidoptera: Noctuidae) under Spodoptera littoralis nucleopolyhedrovirus viral infection.Molecular biology reports · 2024Article
- Article
- Identification of stable housekeeping genes for induced pluripotent stem cells and -derived endothelial cells for drug testing.Scientific reports · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Identification of stably expressed reference genes for expression studies inFrontiers in plant science · 2022Article
- Screening Potential Reference Genes inGenes · 2021Article
- An optimized protocol for stepwise optimization of real-time RT-PCR analysis.Horticulture research · 2021Article
- Article
- Selection of Reference Genes for Quantitative Real-Time PCR inFrontiers in physiology · 2020Article
- Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).Scientific reports · 2019Article
- Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Larvae ofFrontiers in physiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hormones play an important role in the regulation of physiological, developmental and behavioural processes. Many of these mechanisms in insects, however, are still not well understood. One way to investigate hormonal regulation is to analyse gene expression patterns of hormones and their receptors by real-time quantitative polymerase chain reaction (RT-qPCR). This method, however, requires stably expressed reference genes for normalisation. In the present study, we evaluated 11 candidate housekeeping genes as reference genes in samples of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.