Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells.

Torben Johansen, Christina Krabbe, Sissel Ida Schmidt, Alberto Martínez Serrano, Morten Meyer

Abstract read

In one paragraph

Article in Stem cells international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Carbachol, along with calcium, indicates new strategy in neural differentiation of human adipose tissue-derived mesenchymal stem cellsRegenerative therapy · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Torben JohansenDepartment of Cardiovascular and Renal Research, University of Southern Denmark, J.B.Winsløws Vej 21, 5000 Odense C, Denmark.

Christina KrabbeDepartment of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, J.B.Winsløws Vej 21, 5000 Odense C, Denmark.

Sissel Ida SchmidtDepartment of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, J.B.Winsløws Vej 21, 5000 Odense C, Denmark.

Alberto Martínez SerranoDepartment of Molecular Biology and Center of Molecular Biology Severo Ochoa, UAM-CSIC, Campus Cantoblanco, 28049 Madrid, Spain.

Morten MeyerDepartment of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, J.B.Winsløws Vej 21, 5000 Odense C, Denmark.ORCID 0000-0002-9571-1336

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spontaneous cytosolic calcium transients and oscillations have been reported in various tissues of nonhuman and human origin but not in human midbrain-derived stem cells. Using confocal microfluorimetry, we studied spontaneous calcium transients and calcium-regulating mechanisms in a human ventral mesencephalic stem cell line undergoing proliferation and neuronal differentiation. Spontaneous calcium transients were detected in a large fraction of both proliferating (>50%) and differentiating (>55%) cells. We provide evidence for the existence of intracellular calcium stores that respond to muscarinic activation of the cells, having sensitivity for ryanodine and thapsigargin possibly reflecting IP

Identifiers

PMID29201062

DOI10.1155/2017/9605432

PMCPMC5671755

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.