ArticlePharmacognosy magazine
Rapid Quantitative Analysis of Naringenin in the Fruit Bodies of
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6 authors at 1 institution in 1 country.
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Abstract
introductionSanghuang is one of mystical traditional Chinese medicines recorded earliest 2000 years ago, that included various fungi of MATERIALS AND
methodsSample solution was prepared by pretreatment of raw material in two-phase acid hydrolysis and the hydrolysis technology was optimized. After reconstitution, analysis was performed using RP-HPLC-UV. The method validation was investigated and the naringenin content of sample and comparison were determined.
resultsThe naringenin was obtained by two-phase acid hydrolysis method, namely, 10.0 g of raw material was hydrolyzed in 200 mL of 1% sulfuric acid aqueous solution (v/v) and 400 mL of chloroform in oil bath at 110°C for 2 h. Good linearity (
conclusionDue to the high efficiency of sample preparation and high reliability of the HPLC method, it is feasible to use this method for routine analysis of naringenin in the fungus. SUMMARY: A convenient two-phase acid hydrolysis was employed to produce naringenin from raw material, and then an efficient and reliable reversed phase-high performance liquid chromatography-ultra violet method was established to monitor naringenin in the fruit bodies of
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