ArticleSensors and actuators. B, Chemical2017
Electrochemical detection of urinary microRNAs via sulfonamide-bound antisense hybridisation.
Article in Sensors and actuators. B, Chemical, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 62 citations in OpenAlex.
- A rapid, specific, and simple-to-use biosensor for amplification-free determination of microRNA based on electrical potential-assisted and ternary hybridization.Mikrochimica acta · 2025Article
- Advances in electrochemical biosensors employing carbon-based electrodes for detection of biomarkers in diabetes mellitus.ADMET & DMPK · 2024Review
- Urine miRNA signature as potential non-invasive diagnostic biomarker for Hirschsprung's disease.Frontiers in molecular neuroscience · 2024Article
- Analysis of urine cell-free DNA in bladder cancer diagnosis by emerging bioactive technologies and materials.Frontiers in bioengineering and biotechnology · 2024Review
- A label-free electrochemical biosensor based on PBA-Au-MXene QD for miR-122 detection in serum samples.Mikrochimica acta · 2023Article
- Advances in Electrochemical Biosensor Technologies for the Detection of Nucleic Acid Breast Cancer Biomarkers.Sensors (Basel, Switzerland) · 2023Review
- miRNA biomarkers in renal disease.International urology and nephrology · 2022Review
- Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques.Biosensors · 2021Review
- Detection of urinary microRNA biomarkers using diazo sulfonamide-modified screen printed carbon electrodes.RSC advances · 2021Article
- Electrochemical determination of hantavirus using gold nanoparticle-modified graphene as an electrode material and Cu-based metal-organic framework assisted signal generation.Mikrochimica acta · 2021Article
- Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons.Biosensors · 2020Review
- The Translational Potential of Electrochemical DNA-Based Liquid Biopsy.Frontiers in chemistry · 2020Review
- Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis.Scientific reports · 2019Article
- miR-223 and other miRNA's evaluation in chronic kidney disease: Innovative biomarkers and therapeutic tools.Non-coding RNA research · 2019Review
- Graphene oxide with in-situ grown Prussian Blue as an electrochemical probe for microRNA-122.Mikrochimica acta · 2019Article
- MicroRNAs as Urinary Biomarker for Oncocytoma.Disease markers · 2018Article
- Novel Biomarkers in the Diagnosis of Chronic Kidney Disease and the Prediction of Its Outcome.International journal of molecular sciences · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Altered serum and plasma microRNA (miRNA) expression profiles have been observed in numerous human diseases, with a number of studies describing circulating miRNA biomarkers for cancer diagnosis, prognosis and response to treatment, and recruitment to clinical trials for miRNA-based drug therapy already underway. Electrochemical detection of biomarkers in urine has several significant advantages over circulating biomarker analysis including safety, cost, speed and ease of conversion to the point of care environment. Consequently, much current research is underway to identify urinary miRNA biomarkers for a variety of pathologies including prostate and bladder malignancies, and renal disorders. We describe here a robust method capable of electrochemical detection of human urinary miRNAs at femtomolar concentrations using a complementary DNA-modified glassy carbon electrode. A miR-21-specific DNA hybridisation probe was immobilised onto a glassy carbon electrode modified by sulfonic acid deposition and subsequent chlorination. In our pilot system, the presence of synthetic mature miR-21 oligonucleotides increased resistance at the probe surface to electron transfer from the ferricyanide/ferrocyanide electrolyte. Response was linear for 10 nM-10 fM miR-21, with a limit of detection of 20 fM, and detection discriminated between miR-21, three point-mutated miR-21 sequences, and miR-16. We then demonstrated similar sensitivity and reproducibility of miR-21 detection in urine samples from 5 human control subjects. Our protocol provides a platform for future high-throughput screening of miRNA biomarkers in liquid biopsies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.