Evidence map›Paper›PMID 29199992›Full record

ArticleActa crystallographica. Section F, Structural biology communications2017

The Pseudomonas syringae pv. actinidiae chemoreceptor protein F (PscF) periplasmic sensor domain: cloning, purification and X-ray crystallographic analysis.

Tifany Oulavallickal, Jodi L Brewster, James L O McKellar, Michael J Fairhurst, Nicholas A Tenci, Monica L Gerth

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In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Nitric oxide signaling in ctenophores.Frontiers in neuroscience · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tifany OulavallickalDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.
Jodi L BrewsterDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.
James L O McKellarDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.
Michael J FairhurstDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.
Nicholas A TenciDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.
Monica L GerthDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nitrate- and nitrite-sensing (NIT) domains are found associated with a wide variety of bacterial receptors, including chemoreceptors. However, the structure of a chemoreceptor-associated NIT domain has not yet been characterized. Recently, a chemoreceptor named PscF was identified from the plant pathogen Pseudomonas syringae pv. actinidiae that is predicted to contain a periplasmic NIT domain. The PscF sensor domain (PscF-SD; residues 42-332) was cloned into an appropriate expression vector, recombinantly produced in Escherichia coli BL21-Gold(DE3) cells and purified via immobilized metal-affinity and size-exclusion chromatography. Purified PscF-SD was screened for crystallization; the best crystal diffracted to a maximum resolution of 1.46 Å in space group P2

Indexed as

Bacterial ProteinsChemotactic FactorsChromatography, AffinityChromatography, GelCloning, MolecularCrystallography, X-RayNitratesNitritesPeriplasmProtein DomainsPseudomonas syringaeBacterial ProteinsChemotactic FactorsNitratesNitriteschemoreceptorchemotaxisligand-binding domainNIT domainPseudomonas syringaesensor domain

Identifiers

PMID29199992
PMCPMC5713676

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.