ArticleBMC veterinary research2017
Neglected zoonotic agents in cattle abortion: tackling the difficult to grow bacteria.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Bovine infectious abortion: a systematic review and meta-analysis.Frontiers in veterinary science · 2023Pooled it
- Development of adaptive neuro-fuzzy inference systems and a user interface for bovine abortive diseases prediction.Tropical animal health and production · 2026Article
- Immune mechanisms underlying endothelial and placental dysfunction in leptospirosis during pregnancy.Frontiers in cellular and infection microbiology · 2026Review
- Personal biosecurity among livestock producers and veterinarians in Armenia and the Republic of Moldova.Frontiers in veterinary science · 2026Article
- Infectious causes of pregnancy loss in cattle: a review of loss intensity and major reproductive pathogens involved.Animal reproduction · 2026Review
- Pathogen-driven pregnancy loss in dairy cattle: An overview of diagnostic advances and innovations.Open veterinary journal · 2025Review
- Shedding light on risk: Seroprevalence of Q fever among farm animals and workers in Ecuador.One health (Amsterdam, Netherlands) · 2024Article
- Study on fungal contaminants in aborted calves of cattle herds in Iran.Veterinary medicine and science · 2024Article
- Rodent-borne parasites in Qatar: A possible risk at the human-animal-ecosystem interface.One health (Amsterdam, Netherlands) · 2024Article
- Molecular detection of bacterial zoonotic abortive agents from ruminants in Turkey.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014-2020.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Seroprevalence ofHeliyon · 2024Article
- Serological and molecular detection as well as typing ofHeliyon · 2024Article
- Abortion associated with postpartum opportunistic bacterial invasion reduces fertility and induces disturbances of reproductive hormones, hematological profile, and oxidant/antioxidant profiles in dairy cows.Journal of advanced veterinary and animal research · 2023Article
- A systematic review on leptospirosis in cattle: A European perspective.One health (Amsterdam, Netherlands) · 2023Review
- Unconventional Sites for Diagnosis of Leptospirosis in Bovine Anicteric Fetuses.Animals : an open access journal from MDPI · 2023Article
- A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats.Scientific reports · 2023Article
- Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion.Pathogens (Basel, Switzerland) · 2023Article
- Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants.The Onderstepoort journal of veterinary research · 2023Article
- Serological Investigation of BovineArchives of Razi Institute · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundCoxiella burnetii, Chlamydia abortus and Leptospira spp. are difficult to grow bacteria that play a role in bovine abortion, but their diagnosis is hampered by their obligate intracellular lifestyle (C. burnetii, C. abortus) or their lability (Leptospira spp.). Their importance is based on the contagious spread in food-producing animals, but also as zoonotic agents. In Switzerland, first-line routine bacteriological diagnostics in cattle abortions is regulated by national law and includes only basic screening by staining for C. burnetii due to the high costs associated with extended spectrum analysis. The aim of this study was to assess the true occurrence of these zoonotic pathogens in 249 cases of bovine abortion in Switzerland by serology (ELISA for anti-C. burnetii and C. abortus antibodies and microscopic agglutination test for anti-Leptospira spp. antibodies), molecular methods (real-time PCR and sequencing of PCR products of Chlamydiales-positive cases), Stamp's modification of the Ziehl-Neelsen (mod-ZN) stain and, upon availability of material, by histology and immunohistochemistry (IHC).
resultsAfter seroanalysis the prevalence was 15.9% for C. burnetii, 38.5% for C. abortus and 21.4% for Leptospira spp. By real-time PCR 12.1% and 16.9% of the cases were positive for C. burnetii and Chlamydiales, respectively, but only 2.4% were positive for C. burnetii or Chlamydiales by mod-ZN stain. Sequencing of PCR products of Chlamydiales-positive cases revealed C. abortus in 10% of cases and the presence of a mix of Chlamydiales-related bacteria in 5.2% of cases. Pathogenic Leptospira spp. were detected in 5.6% of cases. Inflammatory lesions were present histologically in all available samples which were real-time PCR-positive for Chlamydiales and Leptospira spp. One of 12 real-time PCR-positive cases for C. burnetii was devoid of histological lesions. None of the pathogens could be detected by IHC.
conclusionMolecular detection by real-time PCR complemented by histopathological analysis is recommended to improve definitive diagnosis of bovine abortion cases and determine a more accurate prevalence of these zoonotic pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.