Evidence map›Paper›PMID 29196784›Full record

ArticleArchives of toxicology2018

PARP1 protects from benzo[a]pyrene diol epoxide-induced replication stress and mutagenicity.

Jan M F Fischer, Tabea Zubel, Kirsten Jander, Jelena Fix, Irmela R E A Trussina, Daniel Gebhard, Jörg Bergemann, Alexander Bürkle, Aswin Mangerich

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of toxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PMInternational journal of environmental research and public health · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jan M F FischerMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany.
Tabea ZubelMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany.
Kirsten JanderMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany.
Jelena FixMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany.
Irmela R E A TrussinaMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany.
Daniel GebhardDepartment of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Albstadt, Germany.
Jörg BergemannDepartment of Life Sciences, Albstadt-Sigmaringen University of Applied Sciences, Albstadt, Germany.
Alexander BürkleMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany. alexander.buerkle@uni-konstanz.de.
Aswin MangerichMolecular Toxicology Group, Department of Biology, University of Konstanz, 78457, Konstanz, Germany. aswin.mangerich@uni-konstanz.de.
University of Konstanz · DEAlbstadt-Sigmaringen University · DE

Funding

Deutsche Forschungsgemeinschaft CRC969Universitat Konstanz YSF
6 · The paper itself

Abstract

Poly(ADP-ribosyl)ation (PARylation) is a complex and reversible posttranslational modification catalyzed by poly(ADP-ribose)polymerases (PARPs), which orchestrates protein function and subcellular localization. The function of PARP1 in genotoxic stress response upon induction of oxidative DNA lesions and strand breaks is firmly established, but its role in the response to chemical-induced, bulky DNA adducts is understood incompletely. To address the role of PARP1 in the response to bulky DNA adducts, we treated human cancer cells with benzo[a]pyrene 7,8-dihydrodiol-9,10-epoxide (BPDE), which represents the active metabolite of the environmental carcinogen benzo[a]pyrene [B(a)P], in nanomolar to low micromolar concentrations. Using a highly sensitive LC-MS/MS method, we revealed that BPDE induces cellular PAR formation in a time- and dose-dependent manner. Consistently, PARP1 activity significantly contributed to BPDE-induced genotoxic stress response. On one hand, PARP1 ablation rescued BPDE-induced NAD

Indexed as

7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideAnimalsCell CycleCHO CellsCricetulusDNA AdductsDNA ReplicationHeLa CellsHistonesHumansMutagenicity TestsMutagensNADPoly (ADP-Ribose) Polymerase-1Reactive Oxygen Species7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxidebenzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNADNA AdductsH2AX protein, humanHistonesMutagensNADPARP1 protein, humanPoly (ADP-Ribose) Polymerase-1Reactive Oxygen SpeciesBenzo[a]pyreneBPDEPARP 1Poly(ADP-ribosyl)ationReplication stress

Identifiers

PMID29196784
PMCPMC5866831
OpenAlexW2774703530

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.