ArticleScientific reports2017
Culturomics and Amplicon-based Metagenomic Approaches for the Study of Fungal Population in Human Gut Microbiota.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 105 citations in OpenAlex.
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- Detection of Fungal Translocation in Patients With Alcohol Use Disorder Using a Real-Time PCR Assay.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2026Article
- Clinical performance of nanopore targeted sequencing for diagnosing endophthalmitis.BMC microbiology · 2025Article
- Gut microbiota influencesNew microbes and new infections · 2025Article
- Overheard at a conference: an in-class activity to foster classroom discussion on ethics and experimental systems in microbiology research.Journal of microbiology & biology education · 2025Article
- Retraction: Tobacco smoking affects the salivary gram-positive bacterial population.Frontiers in public health · 2025Article
- Acupuncture Ameliorates Alzheimer's-Like Cognitive Impairment and Pathological Changes via Regulating the Intestinal Fungal Community in APP/PS1 Mice.Neuropsychiatric disease and treatment · 2025Article
- Article
- Multi-marker comparative analysis of 18S, ITS1, and ITS2 primers for human gut mycobiome profiling.Frontiers in bioinformatics · 2025Article
- Retraction: Among live and dead bacteria, the optimization of sample collection and processing remains essential in recovering gut microbiota components.Frontiers in microbiology · 2025Article
- Retraction: Deciphering the urinary microbiota repertoire by culturomics reveals mostly anaerobic bacteria from the gut.Frontiers in microbiology · 2025Article
- Malian Children's Core Gut Mycobiome.Microorganisms · 2024Article
- Fungi and tumors: The role of fungi in tumorigenesis (Review).International journal of oncology · 2024Review
- Bisphenol A exposure affects specific gut taxa and drives microbiota dynamics in childhood obesity.mSystems · 2024Article
- Yeast metagenomics: analytical challenges in the analysis of the eukaryotic microbiome.Microbiome research reports · 2024Review
- Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.Microorganisms · 2023Review
- Abnormal proliferation of gut mycobiota contributes to the aggravation of Type 2 diabetes.Communications biology · 2023Article
- Metagenomic detection of eumycetoma causative agents from households of patients residing in two Sudanese endemic villages in White Nile State.PLoS neglected tropical diseases · 2022Article
- Effect of cryopreservation medium conditions on growth and isolation of gut anaerobes from human faecal samples.Microbiome · 2022Article
- Culturable Yeast Diversity of Grape Berries fromJournal of fungi (Basel, Switzerland) · 2022Article
Corrections and comments
- Retraction · 2024-04-02Concerns/Issues about Human Subject Welfare · Investigation by Journal/Publisher · Lack of IRB/IACUC Approval and/or Compliance · Objections by Author(s) ·
- Retracted
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herein, the mycobiota was characterized in fecal samples from sick patients and healthy subjects, collected from different geographical locations and using both culturomics and amplicon-based metagenomics approaches. Using the culturomics approach, a total of 17,800 fungal colonies were isolated from 14 fecal samples, and resulted in the isolation of 41 fungal species, of which 10 species had not been previously reported in the human gut. Deep sequencing of fungal-directed ITS1 and ITS2 amplicons led to the detection of a total of 142 OTUs and 173 OTUs from the ITS1 and ITS2 regions, respectively. Ascomycota composed the largest fraction of the total OTUs analyzed (78.9% and 68.2% of the OTUs from the ITS1 and ITS2 regions, respectively), followed by Basidiomycota (16.9% and 30.1% of the OTUs from the ITS1 and ITS2 regions, respectively). Interestingly, the results demonstrate that the ITS1/ITS2 amplicon sequencing provides different information about gut fungal communities compared to culturomics, though both approaches complete each other in assessing fungal diversity in fecal samples. We also report higher fungal diversity and abundance in patients compared to healthy subjects. In conclusion, combining both culturomic and amplicon-based metagenomic approaches may be a novel strategy towards analyzing fungal compositions in the human gut.
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