Evidence map›Paper›PMID 29196704›Full record

ArticleScientific reports2017

Repurposing a photosynthetic antenna protein as a super-resolution microscopy label.

Samuel F H Barnett, Andrew Hitchcock, Amit K Mandal, Cvetelin Vasilev, Jonathan M Yuen, James Morby, Amanda A Brindley, Dariusz M Niedzwiedzki, Donald A Bryant, Ashley J Cadby and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Research square · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Samuel F H BarnettDepartment of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, UK.ORCID 0000-0002-3696-3509
Andrew HitchcockDepartment of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, UK.ORCID 0000-0001-6572-434X
Amit K MandalDepartment of Chemistry, Washington University, St. Louis, MO, 63130, USA.
Cvetelin VasilevDepartment of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
Jonathan M YuenDepartment of Chemistry, Washington University, St. Louis, MO, 63130, USA.
James MorbyDepartment of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
Amanda A BrindleyDepartment of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
Dariusz M NiedzwiedzkiDepartment of Chemistry, Washington University, St. Louis, MO, 63130, USA.ORCID 0000-0002-1976-9296
Donald A BryantDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, 16802, USA.
Ashley J CadbyDepartment of Physics and Astronomy, Hicks Building, Hounsfield Road, Sheffield, S3 7RH, UK.
Dewey HoltenDepartment of Chemistry, Washington University, St. Louis, MO, 63130, USA.
C Neil HunterDepartment of Molecular Biology and Biotechnology, Firth Court, Western Bank, Sheffield, S10 2TN, UK. c.n.hunter@sheffield.ac.uk.ORCID 0000-0003-2533-9783
Washington University in St. Louis · USUniversity of Sheffield · GBPennsylvania State University · US

Funding

Biotechnology and Biological Sciences Research Council BB/M000265/1Medical Research Council MR/K015753/1
6 · The paper itself

Abstract

Techniques such as Stochastic Optical Reconstruction Microscopy (STORM) and Structured Illumination Microscopy (SIM) have increased the achievable resolution of optical imaging, but few fluorescent proteins are suitable for super-resolution microscopy, particularly in the far-red and near-infrared emission range. Here we demonstrate the applicability of CpcA, a subunit of the photosynthetic antenna complex in cyanobacteria, for STORM and SIM imaging. The periodicity and width of fabricated nanoarrays of CpcA, with a covalently attached phycoerythrobilin (PEB) or phycocyanobilin (PCB) chromophore, matched the lines in reconstructed STORM images. SIM and STORM reconstructions of Escherichia coli cells harbouring CpcA-labelled cytochrome bd

Indexed as

Bacterial ProteinsLight-Harvesting Protein ComplexesPhotosynthesisPhycobilinsPhycocyaninPhycoerythrinStochastic ProcessesSynechocystisBacterial ProteinsLight-Harvesting Protein ComplexesPhycobilinsPhycocyaninphycocyanobilinPhycoerythrinphycoerythrobilin

Identifiers

PMID29196704
PMCPMC5711914
OpenAlexW2768393853

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.