ArticleProceedings of the National Academy of Sciences of the United States of America2017
Protein kinase C and calcineurin cooperatively mediate cell survival under compressive mechanical stress.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- 3D confinement physically regulates cell cycle progression in budding yeast.Nature communications · 2026Article
- Regulation of the Cell Wall Integrity pathway at the contact site between mating partners in yeast.The Journal of cell biology · 2026Article
- Unraveling the protein kinase C/NDRG1 signaling network in breast cancer.Cell & bioscience · 2024Article
- Mechanical stimulation controls osteoclast function through the regulation of CaCommunications biology · 2023Article
- Donor-Derived Cell-Free DNA for Kidney Allograft Surveillance after Conversion to Belatacept: Prospective Pilot Study.Journal of clinical medicine · 2023Article
- Cip1 tunes cell cycle arrest duration upon calcineurin activation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cell cycle-independent integration of stress signals by Xbp1 promotes Non-G1/G0 quiescence entry.The Journal of cell biology · 2022Article
- The Fission Yeast Cell Integrity Pathway: A Functional Hub for Cell Survival upon Stress and Beyond.Journal of fungi (Basel, Switzerland) · 2021Review
- Crosstalk between the calcineurin and cell wall integrity pathways prevents chitin overexpression in Candida albicans.Journal of cell science · 2021Article
- CWI pathway participated in vegetative growth and pathogenicity through a downstream effector AflRlm1 iniScience · 2021Article
- Application of Double-Strand RNAs Targeting Chitin Synthase, Glucan Synthase, and Protein Kinase ReducesFrontiers in microbiology · 2021Article
- Mechanism of Candida pathogenesis: revisiting the vital drivers.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Mechanical force-induced morphology changes in a human fungal pathogen.BMC biology · 2020Article
- Highly conserved extracellular residues mediate interactions between pore-forming and regulatory subunits of the yeast CaThe Journal of biological chemistry · 2020Article
- TOR complex 2 (TORC2) signaling and the ESCRT machinery cooperate in the protection of plasma membrane integrity in yeast.The Journal of biological chemistry · 2020Article
- Crosstalk and spatiotemporal regulation between stress-induced MAP kinase pathways and pheromone signaling in budding yeast.Cell cycle (Georgetown, Tex.) · 2020Review
- Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5.The Journal of cell biology · 2019Article
- A Toolbox for Organelle Mechanobiology Research-Current Needs and Challenges.Micromachines · 2019Article
- Carbon Catabolite Repression in Yeast is Not Limited to Glucose.Scientific reports · 2019Article
- Ser/Thr protein phosphatases in fungi: structure, regulation and function.Microbial cell (Graz, Austria) · 2019Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells experience compressive stress while growing in limited space or migrating through narrow constrictions. To survive such stress, cells reprogram their intracellular organization to acquire appropriate mechanical properties. However, the mechanosensors and downstream signaling networks mediating these changes remain largely unknown. Here, we have established a microfluidic platform to specifically trigger compressive stress, and to quantitatively monitor single-cell responses of budding yeast in situ. We found that yeast senses compressive stress via the cell surface protein Mid2 and the calcium channel proteins Mid1 and Cch1, which then activate the Pkc1/Mpk1 MAP kinase pathway and calcium signaling, respectively. Genetic analysis revealed that these pathways work in parallel to mediate cell survival. Mid2 contains a short intracellular tail and a serine-threonine-rich extracellular domain with spring-like properties, and both domains are required for mechanosignaling. Mid2-dependent spatial activation of the Pkc1/Mpk1 pathway depolarizes the actin cytoskeleton in budding or shmooing cells, thereby antagonizing polarized growth to protect cells under compressive stress conditions. Together, these results identify a conserved signaling network responding to compressive mechanical stress, which, in higher eukaryotes, may ensure cell survival in confined environments.
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