ArticleJournal of virological methods2018
Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.
Article in Journal of virological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
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- Narrowed host ranges do not constrain future host range expansion in RNA phage Φ6.Journal of virology · 2026Article
- Translational Quantitative Proteomic Assay for Bacteriophages: A New Frontier in Phage Pharmaceutical Development.bioRxiv : the preprint server for biology · 2026Article
- Phage amplification-coupled CRISPR/Cas12a system for selective detection of viableFrontiers in microbiology · 2026Article
- Annexin A1-Targeted d-Peptide-Monomethyl Auristatin E Conjugate Enhanced Antitumor Effect of Monomethyl Auristatin E for Chemotherapy of Prostate Cancer.The Prostate · 2025Article
- A novel miniaturized filamentous phagemid as a gene delivery vehicle to target mammalian cells.Molecular therapy. Nucleic acids · 2025Article
- Evaluating phage lytic activity: from plaque assays to single-cell technologies.Frontiers in microbiology · 2025Review
- Control of lysogeny and antiphage defense by a prophage-encoded kinase-phosphatase module.Nature communications · 2024Article
- Article
- Characterization of selected phages for biocontrol of food-spoilage pseudomonads.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Phage Display Identified Novel Leydig Cell Homing Peptides for Testicular Targeting.ACS pharmacology & translational science · 2024Article
- Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method.PloS one · 2024Article
- Construction and characterization of a novel miniaturized filamentous phagemid for targeted mammalian gene transfer.Microbial cell factories · 2023Article
- Genome-scale top-down strategy to generate viable genome-reduced phages.Nucleic acids research · 2022Article
- A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy.Synthetic and systems biotechnology · 2022Article
- Article
- Evaluation of a direct phage DNA detection-based Taqman qPCR methodology for quantification of phage and its application in rapid ultrasensitive identification of Acinetobacter baumannii.BMC infectious diseases · 2022Article
- An InducibleViruses · 2022Article
- A White Plaque, Associated with Genomic Deletion, Derived from M13KE-Based Peptide Library Is Enriched in a Target-Unrelated Manner during Phage Display Biopanning Due to Propagation Advantage.International journal of molecular sciences · 2022Article
- Quantitative Imaging of Bacteriophage Amplification for Rapid Detection of Bacteria in Model Foods.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7 phages were quantified by TaqMan or SYBR Green qPCR referenced against M13 and T7 DNA standard curves of known concentrations. TaqMan qPCR was capable of quantifying M13 and T7 phage DNA simultaneously with a detection range of 2.75*10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.