ArticleMethods (San Diego, Calif.)2018
Isolation of mammalian stress granule cores for RNA-Seq analysis.
Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 63 citations in OpenAlex.
- Probing the Role of RNA Helicases in Modulating Stress Granules, RNA Localization, and P-Body Docking.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A practical guide to investigating biomolecular condensates: a comment from the plant community.Science China. Life sciences · 2026Review
- BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stress granules promote DNA damage repair through the G3BP1/NAT10/ATF3 axis to facilitate nasopharyngeal carcinoma progression.Oncogene · 2026Article
- Crowding Effects in RNA Interactions.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The G3BP stress-granule proteins reinforce the integrated stress response translation programme.Nature cell biology · 2026Article
- Stress granules promote quiescence by enhancing p21 levels and reducing phospho-Rb.RNA (New York, N.Y.) · 2025Article
- METTL1 promotes cadmium-induced stress granules formation via enhancing translation of G3BP1 and expression of mCell biology and toxicology · 2025Article
- DEAD-Box Helicase 3 Modulates the Non-Coding RNA Pool in Ribonucleoprotein Condensates During Stress Granule Formation.Non-coding RNA · 2025Article
- Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology.Science advances · 2025Article
- Rim4-seeded stress granules connect temperature sensing to meiotic regulation.Nature communications · 2025Article
- Dissecting the stress granule RNA world: dynamics, strategies, and data.RNA (New York, N.Y.) · 2025Review
- ALS-associated FUS mutation reshapes the RNA and protein composition of stress granules.Nucleic acids research · 2024Article
- RNase L-induced bodies sequester subgenomic flavivirus RNAs to promote viral RNA decay.Cell reports · 2024Article
- Mapping subcellular RNA localization with proximity labeling.Acta biochimica et biophysica Sinica · 2024Review
- MNature communications · 2024Article
- DDX6 modulates P-body and stress granule assembly, composition, and docking.The Journal of cell biology · 2024Article
- N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules.EMBO reports · 2024Article
- RNase L-induced bodies sequester subgenomic flavivirus RNAs and re-establish host RNA decay.bioRxiv : the preprint server for biology · 2024Article
- The Role of Heat-Induced Stress Granules in the Blood-Testis Barrier of Mice.International journal of molecular sciences · 2024Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Stress granules are dynamic, conserved non-translating RNA-protein assemblies that form during cellular stress and are related to pathological aggregates in many neurodegenerative diseases. Mammalian stress granules contain stable structures, referred to as "cores" that can be biochemically purified. Herein, we describe a step-by-step guide on how to isolate RNA from stress granule cores for RNA-Seq analysis. We also describe a methodology for validating the RNA-Seq results by single molecule FISH and how to quantify the single molecule FISH results. These protocols provide a starting point for describing the RNA content of stress granules and may assist in the discovery of the assembly mechanisms and functions of stress granules in a variety of biological contexts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.