ArticleThe Review of scientific instruments2017
Multiplexed fluctuation-dissipation-theorem calibration of optical tweezers inside living cells.
Article in The Review of scientific instruments, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Multi-frequency passive and active microrheology with optical tweezers.Scientific reports · 2021Article
- Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events.Micromachines · 2021Article
- Covariance distributions in single particle tracking.Physical review. E · 2021Article
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Authors and funding
5 authors.
Funding
Abstract
In order to apply optical tweezers-based force measurements within an uncharacterized viscoelastic medium such as the cytoplasm of a living cell, a quantitative calibration method that may be applied in this complex environment is needed. We describe an improved version of the fluctuation-dissipation-theorem calibration method, which has been developed to perform in situ calibration in viscoelastic media without prior knowledge of the trapped object. Using this calibration procedure, it is possible to extract values of the medium's viscoelastic moduli as well as the force constant describing the optical trap. To demonstrate our method, we calibrate an optical trap in water, in polyethylene oxide solutions of different concentrations, and inside living fission yeast (S. pombe).
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