ArticleInternational journal for parasitology2018
A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology.
Article in International journal for parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Two- and Three-Dimensional Human Intestinal Tissue Models for Cryptosporidium Infection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Microsporidia infection alters C. elegans lipid levels.PloS one · 2025Article
- Article
- Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1).Parasitology research · 2023Article
- Medicinal plants as a source of antiparasitics: an overview of experimental studies.Pathogens and global health · 2023Article
- A novel, stain-free, natural auto-fluorescent signal, Sig M, identified from cytometric and transcriptomic analysis of infectivity ofFrontiers in cellular and infection microbiology · 2023Article
- Controlled Human Infection Models To Accelerate Vaccine Development.Clinical microbiology reviews · 2022Review
- Transcending Dimensions in Apicomplexan Research: from Two-Dimensional to Three-DimensionalMicrobiology and molecular biology reviews : MMBR · 2022Review
- The Oesophageal Squamous Cell Carcinoma Cell Line COLO-680N Fails to Support SustainedPathogens (Basel, Switzerland) · 2021Article
- First Metabolic Insights intoBiology · 2021Article
- Review
- The Truman Show for protozoan parasites: A review of in vitro cultivation platforms.PLoS neglected tropical diseases · 2021Review
- The Mucosal Innate Immune Response toFrontiers in cellular and infection microbiology · 2021Review
- Organoids and Bioengineered Intestinal Models: Potential Solutions to theMicroorganisms · 2020Review
- Analysis of Long Non-Coding RNA inFrontiers in cellular and infection microbiology · 2020Article
- Two- and Three-Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.Methods in molecular biology (Clifton, N.J.) · 2020Article
- NMR metabolomics reveals effects ofGut pathogens · 2019Article
- Past and future trends of Cryptosporidium in vitro research.Experimental parasitology · 2019Review
- Localization of Fe-S Biosynthesis Machinery in Cryptosporidium parvum Mitosome.The Journal of eukaryotic microbiology · 2018Article
- Recent Breakthroughs and Ongoing Limitations inF1000Research · 2018Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking, mainly due to lack of a long-term culturing system of this parasite. Here, we show that COLO-680N cells infected with two different Cryptosporidium parvum strains produce sufficient infectious oocysts to infect subsequent cultures, showing a substantial fold increase in production, depending on the experiment, over the most optimistic HCT-8 models. Oocyst identity was confirmed using a variety of microscopic- and molecular-based methods. This culturing system will accelerate research on Cryptosporidium and the development of anti-Cryptosporidium drugs.
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Registered trials
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