Evidence map›Paper›PMID 29194950›Full record

ArticleEnvironmental microbiology2018

Multiplex quantitative SILAC for analysis of archaeal proteomes: a case study of oxidative stress responses.

Lana J McMillan, Sungmin Hwang, Rawan E Farah, Jin Koh, Sixue Chen, Julie A Maupin-Furlow

Open access · greenAbstract read
In one paragraph

Article in Environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Journal of bacteriology · 2025
    Review
  10. Article
  11. Bacterial protein acetylation: mechanisms, functions, and methods for study.Frontiers in cellular and infection microbiology · 2024
    Review
  12. Article
  13. Insights into the Lysine Acetylome of the HaloarchaeonAntioxidants (Basel, Switzerland) · 2023
    Article
  14. Article
  15. Genetic Manipulation of Haloferax Species.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  16. Article
  17. Quantitative Mass Spectrometry by SILAC in Haloferax volcanii.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lana J McMillanDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
Sungmin HwangDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
Rawan E FarahDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
Jin KohProteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32611, USA.
Sixue ChenGenetics Institute, University of Florida, Gainesville, FL 32611, USA.
Julie A Maupin-FurlowDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
University of Florida · US

Funding

Ubiquitin-like Small Archaeal Protein Modification (SAMPylation)R01GM057498 · NIGMS · UNIVERSITY OF FLORIDA · PI MAUPIN-FURLOW, JULIE A · 2000 to 2024
$4.9M
NIGMS NIH HHS R01 GM057498
6 · The paper itself

Abstract

Stable isotope labelling of amino acids in cell culture (SILAC) is a quantitative proteomic method that can illuminate new pathways used by cells to adapt to different lifestyles and niches. Archaea, while thriving in extreme environments and accounting for ∼20%-40% of the Earth's biomass, have not been analyzed with the full potential of SILAC. Here, we report SILAC for quantitative comparison of archaeal proteomes, using Haloferax volcanii as a model. A double auxotroph was generated that allowed for complete incorporation of

Indexed as

Oxidative StressProteomeArchaeal ProteinsChromatography, LiquidHaloferax volcaniiIsotope LabelingPeptidesTandem Mass SpectrometryArchaeal ProteinsPeptidesProteome

Identifiers

PMID29194950
PMCPMC5764799
OpenAlexW2774471090

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.