ArticleScientific reports2017
Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- A comprehensive transcriptomic comparison of hepatocyte model systems improves selection of models for experimental use.Communications biology · 2022Pooled it
- Cell and tissue reprogramming: Unlocking a new era in medical drug discovery.Pharmacological reviews · 2025Review
- Comparative transcriptomic and phenotypic analysis of induced pluripotent stem cell hepatocyte-like cells and primary human hepatocytes.Cell and tissue research · 2024Article
- HNF4A guides the MLL4 complex to establish and maintain H3K4me1 at gene regulatory elements.Communications biology · 2024Article
- Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.JHEP reports : innovation in hepatology · 2023Article
- A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Detection of lineage-reprogramming efficiency of tumor cells in a 3D-printed liver-on-a-chip model.Theranostics · 2023Article
- Identification of transcription factors dictating blood cell development using a bidirectional transcription network-based computational framework.Scientific reports · 2022Article
- Probing cell identity hierarchies by fate titration and collision during direct reprogramming.Molecular systems biology · 2022Article
- Repurposing the lineage-determining transcription factor Atoh1 without redistributing its genomic binding sites.Frontiers in cell and developmental biology · 2022Article
- TF-COMB - Discovering grammar of transcription factor binding sites.Computational and structural biotechnology journal · 2022Article
- Preclinical models of idiosyncratic drug-induced liver injury (iDILI): Moving towards prediction.Acta pharmaceutica Sinica. B · 2021Review
- Cell-PermeableInternational journal of molecular sciences · 2021Article
- ANANSE: an enhancer network-based computational approach for predicting key transcription factors in cell fate determination.Nucleic acids research · 2021Article
- Direct conversion of porcine primary fibroblasts into hepatocyte-like cells.Scientific reports · 2021Article
- Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids.Cell systems · 2021Article
- Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver.Nature protocols · 2021Article
- Article
- Generation of metabolically functional hepatocyte-like cells from dedifferentiated fat cells by Foxa2, Hnf4a and Sall1 transduction.Genes to cells : devoted to molecular & cellular mechanisms · 2020Article
- Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, it has been reported that human hepatocyte-like cells can be generated from fibroblasts by direct reprogramming technology. However, the conversion efficiency of human induced hepatocyte-like cells (hiHeps) is not high enough. In addition, comparative analysis with the existing models of hepatocytes, such as human iPS cell-derived hepatocyte-like cells and primary human hepatocytes, has not been sufficiently carried out. In this study, we screened hepatic transcription factors for efficient direct hepatic reprogramming and compared hepatic functions between hiHeps and other existing hepatocyte models. We found that human fibroblasts were efficiently converted into hiHeps by using a combination of ATF5, PROX1, FOXA2, FOXA3, and HNF4A (albumin+/alpha-1 antitrypsin+ cells = 27%, asialoglycoprotein receptor 1+ cells = 22%). The CYP expression levels and CYP activities in hiHeps were higher than those in human iPS cell-derived hepatocyte-like cells, but lower than those in short-term (4 hr) cultured primary human hepatocytes and primary human hepatocytes collected immediately after thawing. These results suggested that functional hiHeps could be efficiently generated by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction. We believe that hiHeps generated by our method will be useful for the drug-discovery activities such as hepatotoxicity screening and drug metabolism tests.
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