ArticleScientific reports2017
Comprehensive high-throughput image analysis for therapeutic efficacy of architecturally complex heterotypic organoids.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- A microfluidic platform for culturing and high-content imaging of adult stem cell-derived organoids.Scientific reports · 2025Article
- Flow cytometry protocol for cell death analysis in glioblastoma organoids: A technical note.PloS one · 2025Article
- Combined physical and biological contributions to radiotherapy enhancement by Lu-based nanoscintillators in pancreatic cancer models.Nanotheranostics · 2025Article
- Dose response test of patient-derived cancer organoids to irradiation.Frontiers in oncology · 2025Article
- Engineering Radiocatalytic Nanoliposomes with Hydrophobic Gold Nanoclusters for Radiotherapy Enhancement.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Neuropilin-2-expressing breast cancer cells mitigate radiation-induced oxidative stress through nitric oxide signaling.The Journal of clinical investigation · 2024Article
- Engineering photodynamics for treatment, priming and imaging.Nature reviews bioengineering · 2024Article
- Nanoscintillator Coating: A Key Parameter That Strongly Impacts Internalization, Biocompatibility, and Therapeutic Efficacy in Pancreatic Cancer Models.Small science · 2024Article
- Methods in cancer research: Assessing therapy response of spheroid cultures by life cell imaging using a cost-effective live-dead staining protocol.Biology methods & protocols · 2024Article
- Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth.PloS one · 2023Article
- 3D Multicellular Tumor Spheroids in a Microfluidic Droplet System for Investigation of Drug Resistance.Polymers · 2022Article
- Eutectic Gallium-Indium Nanoparticles for Photodynamic Therapy of Pancreatic Cancer.ACS applied nano materials · 2022Article
- Assay establishment and validation of a high-throughput organoid-based drug screening platform.Stem cell research & therapy · 2022Article
- Generating Large Numbers of Pancreatic Microtumors on Alginate-Gelatin Hydrogels for Quantitative Imaging of Tumor Growth and Photodynamic Therapy Optimization.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Perfusion Model to Evaluate Response to Photodynamic Therapy in 3D Tumors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Analysis of Treatment Effects on Structurally Complex Microtumor Cultures Using a Comprehensive Image Analysis Procedure.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Spatiotemporal Tracking of Different Cell Populations in Cancer Organoid Models for Investigations on Photodynamic Therapy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Combined Fluorescence and Optoacoustic Imaging for Monitoring Treatments against CT26 Tumors with Photoactivatable Liposomes.Cancers · 2021Article
- Phenotypic screening system using three-dimensional (3D) culture models for natural product screening.The Journal of antibiotics · 2021Review
- Application of LDH assay for therapeutic efficacy evaluation of ex vivo tumor models.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Bioengineered three-dimensional (3D) tumor models that incorporate heterotypic cellular communication are gaining interest as they can recapitulate key features regarding the intrinsic heterogeneity of cancer tissues. However, the architectural complexity and heterogeneous contents associated with these models pose a challenge for toxicological assays to accurately report treatment outcomes. To address this issue, we describe a comprehensive image analysis procedure for structurally complex organotypic cultures (CALYPSO) applied to fluorescence-based assays to extract multiparametric readouts of treatment effects for heterotypic tumor cultures that enables advanced analyses. The capacity of this approach is exemplified on various 3D models including adherent/suspension, mono-/heterocellular cultures and several disease types. The subsequent analysis revealed specific morphological effects of oxaliplatin chemotherapy, radiotherapy, and photodynamic therapy. The procedure can be readily implemented in most laboratories to facilitate high-throughput toxicological screening of pharmaceutical agents and treatment regimens on organotypic cultures of human disease to expedite drug and therapy development.
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Registered trials
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