Evidence map›Paper›PMID 29192254›Full record

ArticleScientific reports2017

Effect of Src Kinase inhibition on Cytochrome c, Smac/DIABLO and Apoptosis Inducing Factor (AIF) Following Cerebral Hypoxia-Ischemia in Newborn Piglets.

Panagiotis Kratimenos, Ioannis Koutroulis, Beamon Agarwal, Stamatios Theocharis, Maria Delivoria-Papadopoulos

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Selective adenosine ASaudi journal of biological sciences · 2021
    Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Panagiotis KratimenosDepartment of Pediatrics, Division of Neonatology, Children's National Medical Center, The George Washington University, School of Medicine and Health Sciences, Washington, DC, USA. panagiotis.kratimenos@childrensnational.org.ORCID 0000-0002-7368-0436
Ioannis KoutroulisDepartment of Pediatrics, Division of Emergency Medicine, Children's National Medical Center, The George Washington University, School of Medicine and Health Sciences, Washington, DC, USA.
Beamon AgarwalDepartment of Hematopathology, Montefiore Medical Center, Bronx, NY, USA.
Stamatios TheocharisFirst Department of Pathology, National and Kapodistrian University of Athens, School of Medicine, Athens, Greece.
Maria Delivoria-PapadopoulosDepartment of Pediatrics, Drexel University College of Medicine, Philadelphia, PA, USA.
Children's National · USDrexel University · USMontefiore Medical Center · USNational and Kapodistrian University of Athens · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have previously shown that cerebral Hypoxia-ischemia (HI) results in activation of Src kinase in the newborn piglet brain. We investigated the regulatory mechanism by which the pre-apoptotic proteins translocate from mitochondria to the cytosol during HI through the Src kinase. Newborn piglets were divided into 3 groups (n = 5/group): normoxic (Nx), HI and HI pre-treated with Src kinase inhibitor PP2 (PP2 + HI). Brain tissue HI was verified by neuropathological analysis and by Adenosine Triphosphate (ATP) and Phosphocreatine (PCr) levels. We used western blots, immunohistochemistry, H&E and biochemical enzyme assays to determine the role of Src kinase on mitochondrial membrane apoptotic protein trafficking. HI resulted in decreased ATP and PCr levels, neuropathological changes and increased levels of cytochrome c, Smac/DIABLO and AIF in the cytosol while their levels were decreased in mitochondria compared to Nx. PP2 decreased the cytosolic levels of pre-apoptotic proteins, attenuated the neuropathological changes and apoptosis and decreased the HI-induced increased activity of caspase-3. Our data suggest that Src kinase may represent a potential target that could interrupt the enzymatic activation of the caspase dependent cell death pathway.

Indexed as

Adenosine TriphosphateAnimalsApoptosisApoptosis Inducing FactorApoptosis Regulatory ProteinsBiomarkersCaspasesCerebral CortexCytochromes cHypoxia-Ischemia, BrainIntracellular Signaling Peptides and ProteinsMitochondriaModels, Biologicalsrc-Family KinasesSwineAdenosine TriphosphateApoptosis Inducing FactorApoptosis Regulatory ProteinsBiomarkersCaspasesCytochromes cIntracellular Signaling Peptides and Proteinssrc-Family Kinases

Identifiers

PMID29192254
PMCPMC5709433
OpenAlexW2770092297

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.