ArticleScientific reports2017
Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Selecting the optimal cell migration assay: fundamentals and practical guidelines.Nature methods · 2026Review
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- Discovering genotype-phenotype relationships with machine learning and the Visual Physiology Opsin Database (VPOD).GigaScience · 2024Article
- Using Optogenetics to Model Cellular Effects of Alzheimer's Disease.International journal of molecular sciences · 2023Review
- A non-canonical Raf function is required for dorsal-ventral patterning during Drosophila embryogenesis.Scientific reports · 2022Article
- Article
- Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos.Developmental cell · 2021Article
- Optogenetic approaches for understanding homeostatic and degenerative processes in Drosophila.Cellular and molecular life sciences : CMLS · 2021Review
- Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems.Annual review of biomedical engineering · 2021Review
- Steering Molecular Activity with Optogenetics: Recent Advances and Perspectives.Advanced biology · 2021Review
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Use of Optogenetic Amyloid-β to Monitor Protein Aggregation inBio-protocol · 2020Article
- Review
- Article
- Parkinson's disease, aging and adult neurogenesis: Wnt/β-catenin signalling as the key to unlock the mystery of endogenous brain repair.Aging cell · 2020Review
- Principles and applications of optogenetics in developmental biology.Development (Cambridge, England) · 2019Review
- From single cells to tissue self-organization.The FEBS journal · 2019Review
- Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications.Genetics · 2019Review
- Blue-Light Receptors for Optogenetics.Chemical reviews · 2018Review
- Illuminating developmental biology with cellular optogenetics.Current opinion in biotechnology · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetics allows precise, fast and reversible intervention in biological processes. Light-sheet microscopy allows observation of the full course of Drosophila embryonic development from egg to larva. Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo. To develop this method, we investigated the regulation of canonical Wnt signaling during anterior-posterior patterning of the Drosophila embryonic epidermis. Cryptochrome 2 (CRY2) from Arabidopsis Thaliana was fused to mCherry fluorescent protein and Drosophila β-catenin to form an easy to visualize optogenetic switch. Blue light illumination caused oligomerization of the fusion protein and inhibited downstream Wnt signaling in vitro and in vivo. Temporal inactivation of β-catenin confirmed that Wnt signaling is required not only for Drosophila pattern formation, but also for maintenance later in development. We anticipate that this method will be easily extendable to other developmental signaling pathways and many other experimental systems.
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