ArticleScientific reports2017
Multilocus DNA barcoding - Species Identification with Multilocus Data.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- eDNA analysis of yard waste samples reveals taxonomical diversity, sequence database limitations, and consistencies across sequencing platforms.Journal of insect science (Online) · 2026Article
- Phylogenetic relationship based on DNA barcodes and comparative analysis of phytochemical contents among Rhynchostylis orchids in Thailand.Scientific reports · 2026Article
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- UnFATE: A Comprehensive Probe Set and Bioinformatics Pipeline for Phylogeny Reconstruction and Multilocus Barcoding of Filamentous Ascomycetes (Ascomycota, Pezizomycotina).Systematic biology · 2025Article
- Taxonomic, molecular and ecological approach reveals high diversity of vector sand flies, varied blood source supply and a high detection rate of Leishmania DNA in Colombian Amazon region.PLoS neglected tropical diseases · 2025Article
- Specimen Identification Through Multilocus Species Tree Constructed From Single-Copy Orthologs (SCOs): A Case Study inEcology and evolution · 2025Article
- A comprehensive DNA barcoding reference database for Plecoptera of Switzerland.Scientific reports · 2024Article
- Speciation in Coastal Basins Driven by Staggered Headwater Captures: Dispersal of a Species Complex, Leporinus bahiensis, as Revealed by Genome-wide SNP Data.Systematic biology · 2023Article
- Phylogenomic analysis provides diagnostic tools for the identification ofEvolutionary applications · 2023Article
- DNA Barcodes ofGenes · 2023Article
- From rivers to ocean basins: The role of ocean barriers and philopatry in the genetic structuring of a cosmopolitan coastal predator.Ecology and evolution · 2023Article
- Super-Mitobarcoding in Plant Species Identification? It Can Work! The Case of Leafy Liverworts Belonging to the GenusInternational journal of molecular sciences · 2022Article
- A targeted capture approach to generating reference sequence databases for chloroplast gene regions.Ecology and evolution · 2022Article
- A high-resolution genome of an euryhaline and eurythermal rhinogoby (Rhinogobius similis Gill 1895).G3 (Bethesda, Md.) · 2022Article
- A customised target capture sequencing tool for molecular identification of Aloe vera and relatives.Scientific reports · 2021Article
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- DNA barcoding of Austrian snow scorpionflies (Mecoptera, Boreidae) reveals potential cryptic diversity inPeerJ · 2021Article
- ACDC, a global database of amphibian cytochrome-b sequences using reproducible curation for GenBank records.Scientific data · 2020Article
- Molecular phylogenetic analysis and morphological reassessments of thief ants identify a new potential case of biological invasions.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species identification using DNA sequences, known as DNA barcoding has been widely used in many applied fields. Current barcoding methods are usually based on a single mitochondrial locus, such as cytochrome c oxidase subunit I (COI). This type of barcoding method does not always work when applied to species separated by short divergence times or that contain introgressed genes from closely related species. Herein we introduce a more effective multi-locus barcoding framework that is based on gene capture and "next-generation" sequencing. We selected 500 independent nuclear markers for ray-finned fishes and designed a three-step pipeline for multilocus DNA barcoding. We applied our method on two exemplar datasets each containing a pair of sister fish species: Siniperca chuatsi vs. Sini. kneri and Sicydium altum vs. Sicy. adelum, where the COI barcoding approach failed. Both of our empirical and simulated results demonstrated that under limited gene flow and enough separation time, we could correctly identify species using multilocus barcoding method. We anticipate that, as the cost of DNA sequencing continues to fall that our multilocus barcoding approach will eclipse existing single-locus DNA barcoding methods as a means to better understand the diversity of the living world.
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