ArticleScientific reports2017
Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 112 citations in OpenAlex.
- Unraveling the role of deuterium in cancer: mechanisms, detection techniques, and therapeutic potential.Molecular diversity · 2026Review
- Ecology-microbiome engineering in Sauce-flavor Baijiu via Single-Cell Raman Spectroscopy.Biodesign research · 2026Review
- µ-Raman and GC-MS to interrogate the heterogeneity of deuterium-label incorporation on the single cell vs. fatty acid isotopologue level.Analytical and bioanalytical chemistry · 2026Article
- Rapid Antifungal Susceptibility Testing by Stimulated Raman Photothermal Imaging of DAnalytical chemistry · 2026Article
- Article
- Rapid culture-free diagnosis of clinical pathogens via integrated microfluidic-Raman micro-spectroscopy.Nature communications · 2025Article
- SIP-SRS Imaging of Cell Wall Synthesis Identifies a Synergy between Micafungin and Amphotericin B.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Application of Rapid Evaporative Ionization Mass Spectrometry (REIMS) to Identify Antimicrobial Resistance in UropathogenicAnalytical chemistry · 2025Article
- Synthetic Plantaricins Derived fromJournal of microbiology and biotechnology · 2025Article
- Analytical techniques and molecular platforms for detection and surveillance of antimicrobial resistance: advancements of the past decade.3 Biotech · 2025Review
- Raman Microspectroscopy to Trace the Incorporation of Deuterium from Labeled (Micro)Plastics into Microbial Cells.Analytical chemistry · 2025Article
- Metabolite microextraction on surface-enhanced Raman scattering nanofibres and DNpj biosensing · 2025Article
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.Science advances · 2024Article
- Single-cell exploration of active phosphate-solubilizing bacteria across diverse soil matrices for sustainable phosphorus management.Nature food · 2024Article
- Sensing the Bactericidal and Bacteriostatic Antimicrobial Mode of Action Using Raman Deuterium Stable Isotope Probing (DSIP) inACS omega · 2024Article
- Raman micro-spectroscopy as a tool to study immunometabolism.Biochemical Society transactions · 2024Review
- Raman Flow Cytometry and Its Biomedical Applications.Biosensors · 2024Review
- Antimicrobial resistance detection methods in water environments: a scoping review.Sustainable microbiology · 2024Review
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.bioRxiv : the preprint server for biology · 2023Article
- Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy.Plant methods · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence and widespread distribution of antimicrobial resistant (AMR) bacteria has led to an increasing concern with respect to potential environmental and public health risks. Culture-independent and rapid identification of AMR bacteria in-situ in complex environments is important in understanding the role of viable but non-culturable and antibiotic persistent bacteria and in revealing potential pathogens without waiting for colony formation. In this study, a culture-independent and non-destructive phenotyping approach, so called Raman Deuterium Stable Isotope Probing (Raman-DIP), was developed to identify AMR bacteria in the River Thames. It is demonstrated that Raman-DIP was able to accurately identify resistant and susceptible bacteria within 24 hours. The work shows that, in the River Thames, the majority of the bacteria (76 ± 2%) were metabolically active, whilst AMR bacteria to carbenicillin, kanamycin and both two antibiotics were 35 ± 5%, 28 ± 3%, 25 ± 1% of the total bacterial population respectively. Raman activated cell ejection (RACE) was applied to isolate single AMR bacteria for the first time, linking AMR phenotype (reistance to antibiotics) and genotype (DNA sequence). The sequences of the RACE sorted cells indicate that they were potential human pathogens Aeromonas sp., Stenotrophomonas sp. and an unculturable bacterium. This work demonstrates Raman-DIP and RACE are effective culture-independent approach for rapid identification of AMR bacteria at the single cell level in their natural conditions.
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