ArticleJournal of structural biology2018
Automated tracing of helical assemblies from electron cryo-micrographs.
Article in Journal of structural biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Helical reconstruction of amyloids in cryoSPARC.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Asymmetric architecture and adaptation of Treponema flagella.Nature communications · 2026Article
- AAV2 Crosslinks Actin Filaments: Implications for AAV Gene Therapy Vector Design.bioRxiv : the preprint server for biology · 2026Article
- Microtubules guide Aurora B substrate geometries for accurate chromosome segregation.Science advances · 2026Article
- Structures of the sheathed flagellum reveal mechanisms of assembly and rotation in Vibrio cholerae.Nature microbiology · 2025Article
- Helicon: Helical parameter determination and 3D reconstruction from one image.Journal of structural biology · 2025Article
- Allosteric control of the bacterial ClpC/ClpP protease and its hijacking by antibacterial peptides.The EMBO journal · 2025Article
- Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages.Bio-protocol · 2024Article
- Semantic segmentation-based detection algorithm for challenging cryo-electron microscopy RNP samples.Frontiers in molecular biosciences · 2024Article
- Structure of a heteropolymeric type 4 pilus from a monoderm bacterium.Nature communications · 2023Article
- Multi-curve fitting and tubulin-lattice signal removal for structure determination of large microtubule-based motors.Journal of structural biology · 2022Article
- High-throughput cryo-EM structure determination of amyloids.Faraday discussions · 2022Article
- Emerging Themes in CryoEM─Single Particle Analysis Image Processing.Chemical reviews · 2022Review
- Structural basis of transposon end recognition explains central features of Tn7 transposition systems.Molecular cell · 2022Article
- Helical reconstruction ofBiochemistry and biophysics reports · 2021Article
- Challenges in sample preparation and structure determination of amyloids by cryo-EM.The Journal of biological chemistry · 2021Review
- Automated picking of amyloid fibrils from cryo-EM images for helical reconstruction with RELION.Journal of structural biology · 2021Article
- Two particle-picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER.Acta crystallographica. Section D, Structural biology · 2020Article
- Structures of cofilin-induced structural changes reveal local and asymmetric perturbations of actin filaments.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Elucidation of the viral disassembly switch of tobacco mosaic virus.EMBO reports · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structure determination of helical specimens commonly requires datasets from thousands of micrographs often obtained by automated cryo-EM data acquisition. Interactive tracing of helical assemblies from such a number of micrographs is labor-intense and time-consuming. Here, we introduce an automated tracing tool MicHelixTrace that precisely locates helix traces from micrographs of rigid as well as very flexible helical assemblies with small numbers of false positives. The computer program is fast and has low computational requirements. In addition to helix coordinates required for a subsequent helical reconstruction work-flow, we determine the persistence length of the polymer ensemble. This information provides a useful measure to characterize mechanical properties of helical assemblies and to evaluate the potential for high-resolution structure determination.
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