ArticlePloS one2017
Campylobacter jejuni transcriptome changes during loss of culturability in water.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Consortium ofAntibiotics (Basel, Switzerland) · 2024Article
- Transcriptome Analysis ofPathogens (Basel, Switzerland) · 2023Article
- Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions.International journal of molecular sciences · 2021Article
- The Effect of Visible Light on Cell Envelope Subproteome duringMicroorganisms · 2021Article
- RevisitingFrontiers in cellular and infection microbiology · 2020Review
- Strain-Specific Differences in Survival ofApplied and environmental microbiology · 2019Article
- Molecular Detection and Epidemiological Features of Selected Bacterial, Viral, and Parasitic Enteropathogens in Stool Specimens from Children with Acute Diarrhea in Thi-Qar Governorate, Iraq.International journal of environmental research and public health · 2019Article
- Retail liver juices enhance the survivability of Campylobacter jejuni and Campylobacter coli at low temperatures.Scientific reports · 2019Article
- Detection and Quantification of Viable but Non-culturableFrontiers in microbiology · 2019Article
- FliW controls growth-phase expression of Campylobacter jejuni flagellar and non-flagellar proteins via the post-transcriptional regulator CsrA.Microbiology (Reading, England) · 2018Article
- Characterization of Swedish Campylobacter coli clade 2 and clade 3 water isolates.MicrobiologyOpen · 2018Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundWater serves as a potential reservoir for Campylobacter, the leading cause of bacterial gastroenteritis in humans. However, little is understood about the mechanisms underlying variations in survival characteristics between different strains of C. jejuni in natural environments, including water.
resultsWe identified three Campylobacter jejuni strains that exhibited variability in their ability to retain culturability after suspension in tap water at two different temperatures (4°C and 25°C). Of the three, strains C. jejuni M1 exhibited the most rapid loss of culturability whilst retaining viability. Using RNAseq transcriptomics, we characterised C. jejuni M1 gene expression in response to suspension in water by analyzing bacterial suspensions recovered immediately after introduction into water (Time 0), and from two sampling time/temperature combinations where considerable loss of culturability was evident, namely (i) after 24 h at 25°C, and (ii) after 72 h at 4°C. Transcript data were compared with a culture-grown control. Some gene expression characteristics were shared amongst the three populations recovered from water, with more genes being up-regulated than down. Many of the up-regulated genes were identified in the Time 0 sample, whereas the majority of down-regulated genes occurred in the 25°C (24 h) sample.
conclusionsVariations in expression were found amongst genes associated with oxygen tolerance, starvation and osmotic stress. However, we also found upregulation of flagellar assembly genes, accompanied by down-regulation of genes involved in chemotaxis. Our data also suggested a switch from secretion via the sec system to via the tat system, and that the quorum sensing gene luxS may be implicated in the survival of strain M1 in water. Variations in gene expression also occurred in accessory genome regions. Our data suggest that despite the loss of culturability, C. jejuni M1 remains viable and adapts via specific changes in gene expression.
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