Evidence map›Paper›PMID 29190498›Full record

ArticleBiomaterials2018

A gapmer aptamer nanobiosensor for real-time monitoring of transcription and translation in single cells.

Shue Wang, Yuan Xiao, Donna D Zhang, Pak Kin Wong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Vasa, a regulator of localized mRNA translation on the spindle.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023
    Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shue WangDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ 85721, USA.
Yuan XiaoDepartment of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ 85721, USA; Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Donna D ZhangDepartment of Pharmacology and Toxicology, The University of Arizona, Tucson, AZ 85721, USA.
Pak Kin WongDepartment of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ 85721, USA; Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA; Department of Mechanical Engineering and Department of Surgery, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address: pak@engr.psu.edu.

Funding

SOUTHWEST ENVIRONMENTAL HEALTH SCIENCES CENTERP30ES006694 · NIEHS · UNIVERSITY OF ARIZONA · PI Nathan J Cherrington · 1994 to 2026
$36.7M
Mechanoregulation of Tissue MorphogenesisDP2OD007161 · OD · UNIVERSITY OF ARIZONA · PI WONG, PAK KIN · 2010 to 2010
$2.3M
Arsenic, Nrf2 and Autophagy Dysfunction in Type II DiabetesR01DK109555 · NIDDK · UNIVERSITY OF ARIZONA · PI ZHANG, DONNA D · 2016 to 2019
$1.4M
NIDDK NIH HHS R01 DK109555NIEHS NIH HHS P30 ES006694NIH HHS DP2 OD007161
6 · The paper itself

Abstract

Transcription and translation are under tight spatiotemporal regulation among cells to coordinate multicellular organization. Methods that allow massively parallel detection of gene expression dynamics at the single cell level are required for elucidating the complex regulatory mechanisms. Here we present a multiplex nanobiosensor for real-time monitoring of protein and mRNA expression dynamics in live cells based on gapmer aptamers and complementary locked nucleic acid probes. Using the multiplex nanobiosensor, we quantified spatiotemporal dynamics of vascular endothelial growth factor A mRNA and protein expressions in single human endothelial cells during microvascular self-organization. Our results revealed distinct gene regulatory processes in the heterogeneous cell subpopulations.

Indexed as

Computer SystemsProtein BiosynthesisSingle-Cell AnalysisTranscription, GeneticAptamers, NucleotideBiosensing TechniquesGene Expression ProfilingHumansHuman Umbilical Vein Endothelial CellsImaging, Three-DimensionalMicrovesselsNanoparticlesPhenotypeRNA, MessengerVascular Endothelial Growth Factor AAptamers, NucleotideRNA, MessengerVascular Endothelial Growth Factor AAptamerGapmerGene expressionLocked nucleic acidNanobiosensor

Identifiers

PMID29190498
PMCPMC5858577

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.