ArticleGlycobiology2018
Distinct reaction mechanisms for hyaluronan biosynthesis in different kingdoms of life.
Article in Glycobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Article
- Antimicrobial Activity Versus Virulence Potential of Hyaluronic Acid: Balancing Advantages and Disadvantages.International journal of molecular sciences · 2025Review
- Insights into substrate binding and utilization by hyaluronan synthase.bioRxiv : the preprint server for biology · 2025Article
- Structural insights into translocation and tailored synthesis of hyaluronan.Nature structural & molecular biology · 2025Article
- Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase.International journal of molecular sciences · 2024Article
- Article
- Hyaluronan synthases; mechanisms, myths, & mysteries of three types of unique bifunctional glycosyltransferases.Glycobiology · 2023Review
- Special Issue: Biomimetic Organic-Inorganic Composites.Materials (Basel, Switzerland) · 2022Article
- Article
- Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications.Materials (Basel, Switzerland) · 2021Review
- Processivity in Bacterial Glycosyltransferases.ACS chemical biology · 2020Review
- Chloroviruses.Viruses · 2019Review
- Priming and elongation of chitin chains: Implications for chitin synthase mechanism.Cell surface (Amsterdam, Netherlands) · 2019Article
- Biosynthesis of Hyaluronic acid polymer: Dissecting the role of sub structural elements of hyaluronan synthase.Scientific reports · 2019Article
- Inherency of Form and Function in Animal Development and Evolution.Frontiers in physiology · 2019Review
- Heterologous overexpression, purification and functional analysis of plant cellulose synthase from green bamboo.Plant methods · 2019Article
- Single-Molecule Analyses Reveal Rhomboid Proteins Are Strict and Functional Monomers in the Membrane.Biophysical journal · 2018Article
- Quaternary structure of the small amino acid transporter OprG fromThe Journal of biological chemistry · 2018Article
- AKR1B1 Upregulation Contributes to Neuroinflammation and Astrocytes Proliferation by Regulating the Energy Metabolism in Rat Spinal Cord Injury.Neurochemical research · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Hyaluronan (HA) is an acidic high molecular weight cell surface polysaccharide ubiquitously expressed by vertebrates, some pathogenic bacteria and even viruses. HA modulates many essential physiological processes and is implicated in numerous pathological conditions ranging from autoimmune diseases to cancer. In various pathogens, HA functions as a non-immunogenic surface polymer that reduces host immune responses. It is a linear polymer of strictly alternating glucuronic acid and N-acetylglucosamine units synthesized by HA synthase (HAS), a membrane-embedded family-2 glycosyltransferase. The enzyme synthesizes HA and secretes the polymer through a channel formed by its own membrane-integrated domain. To reveal how HAS achieves these tasks, we determined the biologically functional units of bacterial and viral HAS in a lipid bilayer environment by co-immunoprecipitation, single molecule fluorescence photobleaching, and site-specific cross-linking analyses. Our results demonstrate that bacterial HAS functions as an obligate homo-dimer with two functional HAS copies required for catalytic activity. In contrast, the viral enzyme, closely related to vertebrate HAS, functions as a monomer. Using site-specific cross-linking, we identify the dimer interface of bacterial HAS and show that the enzyme uses a reaction mechanism distinct from viral HAS that necessitates a dimeric assembly.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.