Evidence map›Paper›PMID 29190022›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Shotgun Sphingolipid Analysis of Human Aqueous Humor.

Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, Sanjoy K Bhattacharya

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Non-alcoholic fatty liver disease: Insights from sphingolipidomics.Biochemical and biophysical research communications · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Anna TrzecieckaBascom Palmer Eye Institute, McKnight Vision Research Center, University of Miami, Miami, FL, USA.
Ulises ArbeloBascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
Arturo BarronBascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
Genea EdwardsBascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
Sruthi SampathkumarDepartment of Ophthalmology, Case Western Reserve University, Cleveland, OH, USA.
Carol TorisDepartment of Ophthalmology, Case Western Reserve University, Cleveland, OH, USA.
Sanjoy K BhattacharyaBascom Palmer Eye Institute, University of Miami, Miami, FL, USA. sbhattacharya@med.miami.edu.

Funding

University of Miami Computational Ocular Genomics Training ProgramT32EY023194 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI SCOTT, WILLIAM K · 2013 to 2017
$661k
NEI NIH HHS T32 EY023194
6 · The paper itself

Abstract

This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Indexed as

Aqueous HumorCeramidesHumansLysophospholipidsSoftwareSpectrometry, Mass, Electrospray IonizationSphingolipidsSphingomyelinsSphingosineTandem Mass SpectrometryCeramidesLysophospholipidsSphingolipidsSphingomyelinsSphingosinesphingosine 1-phosphateAqueous humorCeramideElectrospray ionization-tandem mass spectrometry (ESI-MS/MS)MZmine 2.21Shotgun lipidomicsSphingolipidsSphingomyelinSphingosineSphingosine-1-phosphate

Identifiers

PMID29190022
PMCPMC7545364

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.