Evidence map›Paper›PMID 29189952›Full record

ArticleParasitology research2018

Immuno-proteomic analysis of Trichinella spiralis, T. pseudospiralis, and T. papuae extracts recognized by human T. spiralis-infected sera.

Chalermchai Somboonpatarakun, Rutchanee Rodpai, Pewpan M Intapan, Oranuch Sanpool, Lakkhana Sadaow, Chaisiri Wongkham, Tonkla Insawang, Thidarut Boonmars, Wanchai Maleewong

Abstract read
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In one paragraph

Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Food and waterborne parasitology · 2022
    Article
  2. Review
  3. Article
  4. Application of RecombinantThe American journal of tropical medicine and hygiene · 2019
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chalermchai SomboonpatarakunDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Rutchanee RodpaiDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Pewpan M IntapanDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Oranuch SanpoolDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Lakkhana SadaowDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Chaisiri WongkhamDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Tonkla InsawangKhon Kaen University Research Instrument Center, Research Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Thidarut BoonmarsDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Wanchai MaleewongDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. wanch_ma@kku.ac.th.ORCID http://orcid.org/0000-0002-6726-3759

Funding

Thailand Research Fund TRF Senior Research Scholar Grant, Thailand Research Fund (Grant No. RTA5880001)Thailand Research Fund (TH) TRF Senior Research Scholar Grant, Thailand Research Fund (Grant No. RTA5880001)The Faculty of Medicine, Khon Kaen University The Faculty of Medicine, Khon Kaen University (Grant No. TR57201)The Faculty of Medicine, Khon Kaen University the Research Assistantship, Faculty of Medicine, Khon Kaen University, Thailand (Grant No. AS58302)The Post-Doctoral Training Program from the Research Affairs and Graduate School, Khon Kaen University, Thailand Grant No. 59146
6 · The paper itself

Abstract

The present study explored potentially immunogenic proteins of the encapsulated (Trichinella spiralis) and non-encapsulated (T. pseudospiralis, T. papuae) species within the genus Trichinella. The somatic muscle larval extracts of each species were subjected to immunoblotting analysis using human T. spiralis-infected serum samples. Fifteen reactive bands of all three species were selected for further protein identification by liquid chromatography-tandem mass spectrometry, and their possible functions were ascertained using the gene ontology. Our findings showed immunogenic protein patterns with molecular mass in the range of 33-67 kDa. Proteomic and bioinformatic analysis revealed a wide variety of functions of 17 identified proteins, which are associated with catalytic, binding, and structural activities. Most proteins were involved in cellular and metabolic processes that contribute in the invasion of host tissues and the larval molting processes. The parasite proteins were identified as actin-5C, serine protease, deoxyribonuclease-2, and intermediate filament protein ifa-1. This information may lead to alternative tools for selection of potential diagnostic protein markers or aid in the design of vaccine candidates for prevention and control of Trichinella infection.

Indexed as

AnimalsAntigens, HelminthChromatography, LiquidGerbillinaeHelminth ProteinsHumansImmunoblottingLarvaMusclesOxidation-ReductionProteomicsTrichinellaTrichinella spiralisTrichinellosisAntigens, HelminthHelminth ProteinsImmunoblottingImmunogenic proteinsMass spectrometryMuscle larvaeTrichinellaTrichinellosis

Identifiers

PMID29189952

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.