Evidence map›Paper›PMID 29189890›Full record

ArticleArchives of microbiology2018

Decoding the role of hypothetical protein All3255 of Anabaena PCC7120 in heavy metal stress management in Escherichia coli.

Prashant Kumar Singh, Mengmeng Tang, Sudhir Kumar, Alok Kumar Shrivastava

Abstract read
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In one paragraph

Article in Archives of microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Prashant Kumar SinghState Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004, Henan, China.
Mengmeng TangState Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004, Henan, China.
Sudhir KumarDepartment of Botany, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Alok Kumar ShrivastavaDepartment of Botany, Mahatma Gandhi Central University, Motihari, Bihar, 845401, India. alokshrivastava@mgcub.ac.in.
Banaras Hindu University · INHenan University · CNMahatma Gandhi Central University

Funding

DEPARTMENT OF SCIENCE AND TECHNOLOGY SERB-YOUNG SCIENTIST
6 · The paper itself

Abstract

Cadmium is a non-essential toxic heavy metal for organisms, including plants and cyanobacteria. Cadmium resistance transporters involved in resistance of cells against various toxicants such as drugs and effluxes cytotoxic compounds from cells. However, cadmium resistance-associated protein (CadD) has never been reported from a diazotrophic cyanobacterium Anabaena sp. To test whether the hypothetical protein All3255 of Anabaena sp. PCC7120 a homolog of cadmium resistance-associated protein (CadD) involved in cadmium or heavy metal resistance or not, cloning and heterologous expression analysis of all3255 performed in Escherichia coli BL21 (DE3). Our results revealed that the strain transformed with pGEX-5X-2 + all3255 showed resistant towards not only to cadmium but also other heavy metals such as nickel, copper, zinc, lead and cobalt in addition to arsenic than those of transformed with empty vector (pGEX-5X-2). Furthermore, the results of metal accumulation analysis of these cells unveil a lower accumulation of tested heavy metals in all3255-overexpressing E. coli cells than those transformed with empty vector. This study strongly supports the role of All3255 of Anabaena sp. PCC7120 as a CadD efflux pump of heavy metals in E.coli.

Indexed as

Adaptation, PhysiologicalAmino Acid SequenceAnabaenaArsenicBacterial ProteinsCadmiumCation Transport ProteinsCloning, MolecularCopperEscherichia coliGene ExpressionMicrobial ViabilityPhylogenySoil PollutantsArsenicBacterial ProteinsCadmiumCation Transport ProteinsCopperSoil PollutantsAnabaena sp. PCC7120Cadmium resistance transporter (CadD)Escherichia coli BL21 (DE3)Heavy metalsHypothetical proteinsResistance

Identifiers

PMID29189890
OpenAlexW2772655801

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.