ArticleNature protocols2017
Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using Patch-seq.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
87 citing papers in PubMed.
- An atlas of primate insular cortex reveals a signal-processing strategy in von Economo neurons.Nature cell biology · 2026Article
- DuBois-Reymond prize lecture 2024: Multi-neuron patch-clamp to uncover the neuronal and synaptic physiology in the human neocortex.Pflugers Archiv : European journal of physiology · 2026Article
- Combined statistical-biophysical modeling links ion channel genes to physiology of cortical neuron types.Patterns (New York, N.Y.) · 2025Article
- Linking ion channel gene expression to neuronal firing patterns through a statistical-biophysical model.Patterns (New York, N.Y.) · 2025Article
- Sensory modality-specific wiring of thalamocortical circuits.Nature reviews. Neuroscience · 2025Review
- The emergence of electrical activity in human brain organoids.Stem cell reports · 2025Review
- 2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neurons.bioRxiv : the preprint server for biology · 2025Article
- Targeted single cell expression profiling identifies integrators of sleep and metabolic state.G3 (Bethesda, Md.) · 2025Article
- Article
- Article
- Multimodal single-cell analyses reveal molecular markers of neuronal senescence in human drug-resistant epilepsy.The Journal of clinical investigation · 2025Article
- Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control.Sensors (Basel, Switzerland) · 2025Article
- Molecular logic for cellular specializations that initiate the auditory parallel processing pathways.Nature communications · 2025Article
- Modeling common and rare genetic risk factors of neuropsychiatric disorders in human induced pluripotent stem cells.Schizophrenia research · 2024Review
- Integrated electrophysiological and genomic profiles of single cells reveal spiking tumor cells in human glioma.Cancer cell · 2024Article
- Molecular logic for cellular specializations that initiate the auditory parallel processing pathways.bioRxiv : the preprint server for biology · 2024Article
- Protocol for mapping cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.STAR protocols · 2024Article
- Joint inference of discrete cell types and continuous type-specific variability in single-cell datasets with MMIDAS.bioRxiv : the preprint server for biology · 2024Article
- Glycine-induced activation of GPR158 increases the intrinsic excitability of medium spiny neurons in the nucleus accumbens.Cellular and molecular life sciences : CMLS · 2024Article
- Organization of corticocortical and thalamocortical top-down inputs in the primary visual cortex.Nature communications · 2024Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Neurons exhibit a rich diversity of morphological phenotypes, electrophysiological properties, and gene-expression patterns. Understanding how these different characteristics are interrelated at the single-cell level has been difficult because of the lack of techniques for multimodal profiling of individual cells. We recently developed Patch-seq, a technique that combines whole-cell patch-clamp recording, immunohistochemistry, and single-cell RNA-sequencing (scRNA-seq) to comprehensively profile single neurons from mouse brain slices. Here, we present a detailed step-by-step protocol, including modifications to the patching mechanics and recording procedure, reagents and recipes, procedures for immunohistochemistry, and other tips to assist researchers in obtaining high-quality morphological, electrophysiological, and transcriptomic data from single neurons. Successful implementation of Patch-seq allows researchers to explore the multidimensional phenotypic variability among neurons and to correlate gene expression with phenotype at the level of single cells. The entire procedure can be completed in ∼2 weeks through the combined efforts of a skilled electrophysiologist, molecular biologist, and biostatistician.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.