ArticleNature protocols2017
Production of knock-in mice in a single generation from embryonic stem cells.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Type I IFN dynamics orchestrate protective ICAM1iScience · 2026Article
- Genetically engineered ESC-derived embryos reveal Vinculin-dependent force responses required for mammalian neural tube closure.bioRxiv : the preprint server for biology · 2025Article
- Postsynaptic competition between calcineurin and PKA regulates mammalian sleep-wake cycles.Nature · 2024Article
- Genetic recording of in vivo cell proliferation by ProTracer.Nature protocols · 2023Review
- Stem Cell-Based Therapeutic Approaches in Genetic Diseases.Advances in experimental medicine and biology · 2023Article
- A growing understanding of the role of muscarinic receptors in the molecular pathology and treatment of schizophrenia.Frontiers in cellular neuroscience · 2023Review
- Distinct phosphorylation states of mammalian CaMKIIβ control the induction and maintenance of sleep.PLoS biology · 2022Article
- Towards organism-level systems biology by next-generation genetics and whole-organ cell profiling.Biophysical reviews · 2021Review
- Rapid and easy-to-use ES cell manipulation device with a small groove near culturing wells.BMC research notes · 2020Article
- Article
- Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice.Animal cells and systems · 2020Article
- Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells.American journal of translational research · 2020Article
- Molecular Mechanisms of REM Sleep.Frontiers in neuroscience · 2019Review
- Easy and efficient production of completely embryonic-stem-cell-derived mice using a micro-aggregation device.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The system-level identification and analysis of molecular networks in mammals can be accelerated by 'next-generation' genetics, defined as genetics that does not require crossing of multiple generations of animals in order to achieve the desired genetic makeup. We have established a highly efficient procedure for producing knock-in (KI) mice within a single generation, by optimizing the genome-editing protocol for KI embryonic stem (ES) cells and the protocol for the generation of fully ES-cell-derived mice (ES mice). Using this protocol, the production of chimeric mice is eliminated, and, therefore, there is no requirement for the crossing of chimeric mice to produce mice that carry the KI gene in all cells of the body. Our procedure thus shortens the time required to produce KI ES mice from about a year to ∼3 months. Various kinds of KI ES mice can be produced with a minimized amount of work, facilitating the elucidation of organism-level phenomena using a systems biology approach. In this report, we describe the basic technologies and protocols for this procedure, and discuss the current challenges for next-generation mammalian genetics in organism-level systems biology studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.