ArticleJournal of mammary gland biology and neoplasia2017
Lipid Droplet Fusion in Mammary Epithelial Cells is Regulated by Phosphatidylethanolamine Metabolism.
Article in Journal of mammary gland biology and neoplasia, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- The Role of ATG8 in Promoting Lipid Accumulation in the Oleaginous FungusJournal of fungi (Basel, Switzerland) · 2026Article
- Milk Fat Globule Membrane: Structural Organization, Bioactive Constituents, and Therapeutic Applications.Foods (Basel, Switzerland) · 2026Review
- Rumen-Plasma-Milk Metabolomics Profiling Revealed Metabolic Alterations Associated with Milk Fat Synthesis in Chinese Holstein Cows.Animals : an open access journal from MDPI · 2026Article
- Ferroptosis in bovine mastitis: multidimensional mechanisms, stratified assessment, and intervention prospects.Frontiers in veterinary science · 2026Review
- Quantifying the Activation Barrier for Phospholipid Monolayer Fusion Governing Lipid Droplet Coalescence.International journal of molecular sciences · 2025Article
- Phosphatidylcholine Cytidine Transferase α (CCTα) Affects LD Formation Through Fusion and Lipophagy in Bovine Mammary Epithelial Cells.International journal of molecular sciences · 2025Article
- The Stressogenic Impact of Bacterial Secretomes Is Modulated by the Size of the Milk Fat Globule Used as a Substrate.Foods (Basel, Switzerland) · 2024Article
- Cellular communication through extracellular vesicles and lipid droplets.Journal of extracellular biology · 2023Review
- Lipid Droplets and Their Participation in Zika Virus Infection.International journal of molecular sciences · 2022Review
- Entourage effect for phenolic compounds on production and metabolism of mammary epithelial cells.Heliyon · 2022Article
- Pistacia lentiscus extract enhances mammary epithelial cells' productivity by modulating their oxidative status.Scientific reports · 2020Article
- Acute exercise in mice transiently remodels the hepatic lipidome in an intensity-dependent manner.Lipids in health and disease · 2020Article
- Milk fat globule size development in the mammary epithelial cell: a potential role for ether phosphatidylethanolamine.Scientific reports · 2020Article
- Investigation on the suitability of milk-derived primary bovine mammary epithelial cells grown on permeable membrane supports as an in vitro model for lactation.In vitro cellular & developmental biology. Animal · 2020Article
- Progesterone Regulation of Milk Fat Globule Size Is VLDL Dependent.Frontiers in endocrinology · 2020Article
- Knockout of butyrophilin subfamily 1 member A1 (Journal of animal science and biotechnology · 2020Article
- Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation.Cell reports · 2019Article
- Symposium review: Intravital imaging of the lactating mammary gland in live mice reveals novel aspects of milk-lipid secretion.Journal of dairy science · 2019Article
- Compositional Dynamics of the Milk Fat Globule and Its Role in Infant Development.Frontiers in pediatrics · 2018Review
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammary epithelial cells (MEC) secrete fat in the form of milk fat globules (MFG) which are found in milk in diverse sizes. MFG originate from intracellular lipid droplets, and the mechanism underlying their size regulation is still elusive. Two main mechanisms have been suggested to control lipid droplet size. The first is a well-documented pathway, which involves regulation of cellular triglyceride content. The second is the fusion pathway, which is less-documented, especially in mammalian cells, and its importance in the regulation of droplet size is still unclear. Using biochemical and molecular inhibitors, we provide evidence that in MEC, lipid droplet size is determined by fusion, independent of cellular triglyceride content. The extent of fusion is determined by the cell membrane's phospholipid composition. In particular, increasing phosphatidylethanolamine (PE) content enhances fusion between lipid droplets and hence increases lipid droplet size. We further identified the underlying biochemical mechanism that controls this content as the mitochondrial enzyme phosphatidylserine decarboxylase; siRNA knockdown of this enzyme reduced the number of large lipid droplets threefold. Further, inhibition of phosphatidylserine transfer to the mitochondria, where its conversion to PE occurs, diminished the large lipid droplet phenotype in these cells. These results reveal, for the first time to our knowledge in mammalian cells and specifically in mammary epithelium, the missing biochemical link between the metabolism of cellular complex lipids and lipid-droplet fusion, which ultimately defines lipid droplet size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.