ArticleMolecular genetics and genomics : MGG2018
Mapping causal mutations by exome sequencing in a wheat TILLING population: a tall mutant case study.
Article in Molecular genetics and genomics : MGG, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 85 citations in OpenAlex.
- Advances in mutant characterization for detecting causal mutations in crop plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Novel genome editing approaches to manipulate apical meristem activity for crop yield.Frontiers in plant science · 2026Review
- Durum wheat mutants with enhanced disease resistance to stripe rust show differential responses to other fungal diseases.Molecular breeding : new strategies in plant improvement · 2025Article
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Genetic analyses and prediction for lodging‑related traits in a diverse Iranian hexaploid wheat collection.Scientific reports · 2024Article
- Dissection of a novel major stable QTL on chromosome 7D for grain hardness and its breeding value estimation in bread wheat.Frontiers in plant science · 2024Article
- Identification and high-resolution mapping of a novel tiller number gene (tin6) by combining forward genetics screen and MutMap approach in bread wheat.Functional & integrative genomics · 2023Article
- Genetic approaches to exploit landraces for improvement ofFrontiers in plant science · 2023Review
- Genome-wide association mapping and genomic prediction of agronomical traits and breeding values in Iranian wheat under rain-fed and well-watered conditions.BMC genomics · 2022Article
- Fine Mapping of Stripe-Rust-Resistance GenePlants (Basel, Switzerland) · 2022Article
- Utility of Triti-Map for bulk-segregated mapping of causal genes and regulatory elements in Triticeae.Plant communications · 2022Article
- Evaluation of nine statistics to identify QTLs in bulk segregant analysis using next generation sequencing approaches.BMC genomics · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Random mutagenesis in vegetatively propagated crops: opportunities, challenges and genome editing prospects.Molecular biology reports · 2022Article
- Effects of the different waxy proteins on starch biosynthesis, starch physicochemical properties and Chinese noodle quality in wheat.Molecular breeding : new strategies in plant improvement · 2022Article
- Whole-exome sequencing of selected bread wheat recombinant inbred lines as a useful resource for allele mining and bulked segregant analysis.Frontiers in genetics · 2022Article
- Genetic Analysis of Adult Plant Resistance to Stripe Rust in Common Wheat Cultivar "Pascal".Frontiers in plant science · 2022Article
- A Multi-Omics Approach for Rapid Identification of Large Genomic Lesions at theFrontiers in plant science · 2022Article
- Whole Exome Sequencing-Based Identification of a Novel Gene Involved in Root Hair Development in Barley (International journal of molecular sciences · 2021Article
- Delaying or delivering: identification of novel NAM-1 alleles that delay senescence to extend wheat grain fill duration.Journal of experimental botany · 2021Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
Forward genetic screens of induced mutant plant populations are powerful tools to identify genes underlying phenotypes of interest. Using traditional techniques, mapping causative mutations from forward screens is a lengthy, multi-step process, requiring the identification of a broad genetic region followed by candidate gene sequencing to characterize the causal variant. Mapping by whole genome sequencing accelerates the identification of causal mutations by simultaneously defining a mapping region and providing information on the induced genetic variants. In wheat, although the availability of a high-quality draft genome assembly facilitates mapping and mutation calling, whole genome resequencing remains prohibitively expensive due to its large genome. In the current study, we used exome sequencing as a complexity reduction strategy to detect mutations associated with a target phenotype. In a segregating wheat EMS population, we identified a clear peak region on chromosome arm 4BS associated with increased plant height. Although none of the significant SNPs seemed causative for the mutant phenotype, they were sufficient to identify a linked ~ 1.9 Mb deletion encompassing nine genes. These genes included Rht-B1, which is known to have a strong effect on plant height and is a strong candidate for the observed phenotype. We performed simulation experiments to determine the impacts of sequencing depth and bulk size and discuss the importance of considering each factor when designing mapping-by-sequencing experiments in wheat. This approach can accelerate the identification of candidate causal point mutations or linked deletions underlying important phenotypes.
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