Evidence map›Paper›PMID 29188149›Full record

ArticleApplications in plant sciences2017

Isolation and characterization of microsatellite primers for the critically endangered shrub

Janet M Anthony, Richard J N Allcock, Mark P Dobrowolski, Siegfried L Krauss

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Article in Applications in plant sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Janet M AnthonyKings Park and Botanic Garden, Botanic Garden and Parks Authority, Fraser Avenue, West Perth, 6005 Western Australia, Australia.
Richard J N AllcockLotterywest State Biomedical Facility: Genomics, School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia 6009, Australia.
Mark P DobrowolskiIluka Resources Limited, Level 23, 140 St. Georges Terrace, Perth, Western Australia 6000, Australia.
Siegfried L KraussKings Park and Botanic Garden, Botanic Garden and Parks Authority, Fraser Avenue, West Perth, 6005 Western Australia, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyMicrosatellite markers were developed for population genetic analysis in the rare shrub METHODS AND

resultsWe generated ca. 2.5 million sequence reads using a Personal Genome Machine semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80-480 bp. From these, 30 primer pairs were selected and screened using PCR; of these, 16 loci were found to be polymorphic, four loci were monomorphic, and 10 loci did not amplify reliably for

conclusionsThese markers will be used to characterize population genetic variation, spatial genetic structure, mating system parameters, and dispersal to aid in the management and conservation of the rare shrub

Indexed as

Astroloma xerophyllumEricaceaemicrosatellite primersshotgun sequencingStyphelia longissima.

Identifiers

PMID29188149
PMCPMC5703184

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.