ArticleMicrobes and environments2017
A Simple and Efficient RNA Extraction Method from Deep-Sea Hydrothermal Vent Chimney Structures.
Article in Microbes and environments, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Different Ferromanganese Concretion Morphologies Host Distinct Microbial Communities and Metal Accumulation Patterns in the Baltic Sea.Environmental microbiology · 2026Article
- Environmental Adaptation Strategies of Deep-Sea Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys.Advanced biology · 2025Article
- Article
- Novel isolates of hydrogen-oxidizing chemolithoautotrophicmSystems · 2024Article
- Article
- A Simple and Effective Method for Solid Medium Cultivation of Strictly Hydrogen- and Sulfur-oxidizing Chemolithoautotrophs Predominant in Deep-sea Hydrothermal Fields.Microbes and environments · 2023Article
- Recent Topics on Deep-Sea Microbial Communities in Microbes and Environments.Microbes and environments · 2019Article
- Cultivation Renaissance in the Post-Metagenomics Era: Combining the New and Old.Microbes and environments · 2019Article
- Enrichment and Genomic Characterization of a NFrontiers in bioengineering and biotechnology · 2018Article
- Deepest and hottest hydrothermal activity in the Okinawa Trough: the Yokosuka site at Yaeyama Knoll.Royal Society open science · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-based microbiological analyses, e.g., transcriptome and reverse transcription-quantitative PCR, require a relatively large amount of high quality RNA. RNA-based analyses on microbial communities in deep-sea hydrothermal environments often encounter methodological difficulties with RNA extraction due to the presence of unique minerals in and the low biomass of samples. In the present study, we assessed RNA extraction methods for deep-sea vent chimneys that had complex mineral compositions. Mineral-RNA adsorption experiments were conducted using mock chimney minerals and Escherichia coli total RNA solution, and showed that detectable RNA significantly decreased possibly due to adsorption onto minerals. This decrease in RNA was prevented by the addition of sodium tripolyphosphate (STPP), deoxynucleotide triphosphates (dNTPs), salmon sperm DNA, and NaOH. The addition of STPP was also effective for RNA extraction from the mixture of E. coli cells and mock chimney minerals when TRIzol reagent and the RNeasy column were used, but not when the RNeasy PowerSoil total RNA kit was used. A combination of STPP, TRIzol reagent, the RNeasy column, and sonication resulted in the highest RNA yield from a natural chimney. This indirect extraction procedure is simple, rapid, inexpensive, and may be used for large-scale RNA extraction.
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