ArticleBMC research notes2017
Doxycycline induces dysbiosis in female C57BL/6NCrl mice.
Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Association of Antibiotic Use and New-Onset ICD Coding of Geographic Atrophy.Investigative ophthalmology & visual science · 2026Article
- Repurposing of doxycycline as a novel therapeutic avenue for management of parkinson's disease.Inflammopharmacology · 2026Review
- Modeling Obesity and Weight Loss in Mice Using Novel AAV Approaches.Obesity (Silver Spring, Md.) · 2026Article
- Gut dysbiosis in a murine model of cutaneous lupus erythematosus correlates with antigen-specific T cells and antigen-presenting cells in skin.Scientific reports · 2026Article
- Evaluating Effects of Antibiotics Across Preclinical Models of the Human Gastrointestinal Microbiota.MicrobiologyOpen · 2025Article
- Assessment of Antimicrobial Therapy in Eradicating Chlamydia muridarum in Research Mice: Immune Status and Its Impact on Outcomes.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Article
- Doxycycline for transgene control disrupts gut microbiome diversity without compromising acute neuroinflammatory response.Journal of neuroinflammation · 2024Article
- Mycobacterial Genetic Technologies for Probing the Host-Pathogen Microenvironment.Infection and immunity · 2023Review
- Tetracycline-Inducible and Reversible Stable Gene Expression in Human iPSC-Derived Neural Progenitors and in the Postnatal Mouse Brain.Current protocols · 2023Article
- Doxycycline Attenuates Pig Intestinal Microbial Interactions and Changes Microbial Metabolic Pathways.Animals : an open access journal from MDPI · 2023Article
- New regulators of the tetracycline-inducible gene expression system identified by chemical and genetic screens.FEBS open bio · 2022Article
- Reemergence of the Murine Bacterial PathogenComparative medicine · 2022Article
- Intestinal Epithelial Regeneration in Response to Ionizing Irradiation.Journal of visualized experiments : JoVE · 2022Article
- Tetracycline response element driven Cre causes ectopic recombinase activity independent of transactivator element.Molecular metabolism · 2022Article
- Effect of Doxycycline in Decreasing the Severity ofAntibiotics (Basel, Switzerland) · 2022Article
- Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats.Nutrients · 2021Article
- Gut Microbiota in Military International Travelers with Doxycycline Malaria Prophylaxis: Towards the Risk of a Simpson Paradox in the Human Microbiome Field.Pathogens (Basel, Switzerland) · 2021Article
- The gut microbiome of laboratory mice: considerations and best practices for translational research.Mammalian genome : official journal of the International Mammalian Genome Society · 2021Review
- Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens.Frontiers in microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveThis study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. Doxycycline is a common effector for control of gene expression using the tet-inducible system in transgenic mice. The effect of oral doxycycline on murine gut microbiota has not been reported. We evaluated the effect of doxycycline treatment by sequencing the V4 hypervariable region of the 16S rRNA gene from fecal samples collected during a 4 week course of treatment at a dose of 2 mg/ml in the drinking water.
resultsThe fecal microbiota of treated animals were distinct from control animals; the decreased richness and diversity were characterized primarily by Bacteroides sp. enrichment. These effects persisted when the treatment was temporarily discontinued for 1 week. These data suggest that doxycycline treatment can induce significant dysbiosis, and its effects should be considered when used in animal models that are or maybe sensitive to perturbation of the gut microbiota.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.