ArticleNature2017
Genetically programmed chiral organoborane synthesis.
Article in Nature, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
84 citing papers in PubMed, 300 citations in OpenAlex.
- Directed evolution of an enantiocomplementary SNature communications · 2026Article
- Enantiodivergent Carbon-Phosphorus Bond Formation via Enzymatic Carbene Insertion.Journal of the American Chemical Society · 2026Article
- SynFit: Synergistic Contrastive Learning for Multi-Objective Protein Fitness Prediction and Optimization.bioRxiv : the preprint server for biology · 2026Article
- PCA-Based Database Mining Enables the Discovery of Bacterial Carbene Transferases for Stereodivergent Cyclopropanation.Angewandte Chemie (International ed. in English) · 2026Article
- A high-throughput biocatalytic platform for screening isomeric kainoid natural products.Cell reports. Physical science · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Catalytic asymmetric constructions of nitrogen, boron and carbon continuous stereogenic centers.Nature communications · 2025Article
- A High-Throughput Biocatalytic Platform for Screening Isomeric Kainoid Natural Products.bioRxiv : the preprint server for biology · 2025Article
- Article
- Metabolic Engineering of the 5-Aminolevulinate Biosynthetic Pathway in E. coli Improves Efficiency of Hemoprotein-Based Biocatalysis.Angewandte Chemie (International ed. in English) · 2025Article
- Creating novel metabolic pathways by protein engineering for bioproduction.Trends in biotechnology · 2025Review
- The direct expression of apo heme proteins: A general strategy to obtain designer heme proteins with abiological cofactors.Methods in enzymology · 2025Article
- Activity of Organoboron Compounds against Biofilm-Forming Pathogens.Antibiotics (Basel, Switzerland) · 2024Review
- Spiers Memorial Lecture: Engineering biocatalysts.Faraday discussions · 2024Article
- Machine learning-guided co-optimization of fitness and diversity facilitates combinatorial library design in enzyme engineering.Nature communications · 2024Article
- Enhanced Sequence-Activity Mapping and Evolution of Artificial Metalloenzymes by Active Learning.ACS central science · 2024Article
- Review
- Current status and emerging frontiers in enzyme engineering: An industrial perspective.Heliyon · 2024Review
- Mechanism of Asymmetric Homologation of Alkenylboronic Acids with CFThe Journal of organic chemistry · 2024Article
- Reaction engineering blocks ether cleavage for synthesizing chiral cyclic hemiacetals catalyzed by unspecific peroxygenase.Nature communications · 2024Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recent advances in enzyme engineering and design have expanded nature's catalytic repertoire to functions that are new to biology. However, only a subset of these engineered enzymes can function in living systems. Finding enzymatic pathways that form chemical bonds that are not found in biology is particularly difficult in the cellular environment, as this depends on the discovery not only of new enzyme activities, but also of reagents that are both sufficiently reactive for the desired transformation and stable in vivo. Here we report the discovery, evolution and generalization of a fully genetically encoded platform for producing chiral organoboranes in bacteria. Escherichia coli cells harbouring wild-type cytochrome c from Rhodothermus marinus (Rma cyt c) were found to form carbon-boron bonds in the presence of borane-Lewis base complexes, through carbene insertion into boron-hydrogen bonds. Directed evolution of Rma cyt c in the bacterial catalyst provided access to 16 novel chiral organoboranes. The catalyst is suitable for gram-scale biosynthesis, providing up to 15,300 turnovers, a turnover frequency of 6,100 h
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.